Determination of Pulchinenoside B<sub>4</sub> in Baitouweng Decoction by HPLC-ELSD

LIU Li-mei; BAI Dong; WANG Rui-hai

doi:10.13422/j.cnki.syfjx.2011.02.026

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Determination of Pulchinenoside B₄ in Baitouweng Decoction by HPLC-ELSD

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- Determination of Pulchinenoside B₄ in Baitouweng Decoction by HPLC-ELSD
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 2, Pages: 70-72(2011)
- 作者机构：
  
  中国中医科学院基础理论研究所
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2011.02.026
  CLC： R284.1
- Published：2011
- 稿件说明：
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[1]刘丽梅,柏冬,王瑞海.HPLC-ELSD联用检测白头翁汤中白头翁皂苷B_4的含量[J].中国实验方剂学杂志,2011,17(02):70-72.

LIU Li-mei, BAI Dong, WANG Rui-hai. Determination of Pulchinenoside B₄ in Baitouweng Decoction by HPLC-ELSD[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(2): 70-72.
[1]刘丽梅,柏冬,王瑞海.HPLC-ELSD联用检测白头翁汤中白头翁皂苷B_4的含量[J].中国实验方剂学杂志,2011,17(02):70-72. DOI： 10.13422/j.cnki.syfjx.2011.02.026.

LIU Li-mei, BAI Dong, WANG Rui-hai. Determination of Pulchinenoside B₄ in Baitouweng Decoction by HPLC-ELSD[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(2): 70-72. DOI： 10.13422/j.cnki.syfjx.2011.02.026.

摘要

目的:建立HPLC-ELSD检测白头翁汤中白头翁皂苷B4含量的方法。方法:采用Agilent Ecilpse XDB-C18色谱柱（4.6 mm×250 mm

5μm）

以乙腈-水（28∶72）为流动相;ELSD漂移管温度100℃

雾化气体（空气）流速2.8 L·min-1。结果:白头翁皂苷B4在1.156～10.404μg线性关系良好

回收率为98.9%（n=5）

RSD 0.7%。结论:本法准确、灵敏、重复性好

为白头翁汤的质量控制提供了依据。

Abstract

Objective:To establish a method for the deter mination of pulchinenoside B4 in Baitouweng decoction by HPLC-ELSD.Method: A HPLC methods detected by HPLC-ELSD has been developed: Agilent Ecilpse XDB-C18 column（4.6 mm × 250 mm

5 μm） with mobile phase of acetonitrile and water（28∶72）;The temperature of the drift tube was set at 100 ℃

the flow rate of gas at 2.8 L·min-1.Result:pulchinenoside B4 in Baitouweng decoction could be deter mined by HPLC-ELSD.The line arrange of pulchinenoside B4 was 1.156-10.404 μg.The recovery was 98.9%（n = 5

RSD 0.7%）.Conclusion:The method is simple and accurate

with good repeatability

and can be used for deter mination of pulchinenoside B4 in Baitouweng decoction.

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Related Author

LIU Li-mei

BAI Dong

WANG Rui-hai

DUAN Haohan

JIA Hao

LEI Jingwei

YANG Chunjing

XIE Caixia

Related Institution

Henan Engineering Research Center for Quality Control and Evaluation of Traditional Chinese Medicine（TCM），School of Pharmacy，Henan University of Chinese Medicine

Luohe Medical College

Third Affiliated Hospital of Henan University of Chinese Medicine

Tianjin University of Traditional Chinese Medicine

Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences

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Determination of Pulchinenoside B4 in Baitouweng Decoction by HPLC-ELSD

DOI：10.13422/j.cnki.syfjx.2011.02.026

摘要

Abstract

关键词

Keywords

references

Determination of Pulchinenoside B₄ in Baitouweng Decoction by HPLC-ELSD