Determination of Five Anthraquinone Derivatives in Niuhuang Xiaoyan Tablets from Different Pharmaceutical Factories by HPLC

XIA Cong-long; ZHOU Nong; ZHONG Jia

doi:10.13422/j.cnki.syfjx.2011.02.030

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Determination of Five Anthraquinone Derivatives in Niuhuang Xiaoyan Tablets from Different Pharmaceutical Factories by HPLC

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- Determination of Five Anthraquinone Derivatives in Niuhuang Xiaoyan Tablets from Different Pharmaceutical Factories by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 2, Pages: 83-86(2011)
- 作者机构：
  
  大理学院药学院
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2011.02.030
  CLC： R286
- Published：2011
- 稿件说明：
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[1]夏从龙,周浓,种佳.HPLC测定不同厂家牛黄消炎片中5种蒽醌类衍生物的含量[J].中国实验方剂学杂志,2011,17(02):83-86.

XIA Cong-long, ZHOU Nong, ZHONG Jia. Determination of Five Anthraquinone Derivatives in Niuhuang Xiaoyan Tablets from Different Pharmaceutical Factories by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(2): 83-86.
[1]夏从龙,周浓,种佳.HPLC测定不同厂家牛黄消炎片中5种蒽醌类衍生物的含量[J].中国实验方剂学杂志,2011,17(02):83-86. DOI： 10.13422/j.cnki.syfjx.2011.02.030.

XIA Cong-long, ZHOU Nong, ZHONG Jia. Determination of Five Anthraquinone Derivatives in Niuhuang Xiaoyan Tablets from Different Pharmaceutical Factories by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(2): 83-86. DOI： 10.13422/j.cnki.syfjx.2011.02.030.

摘要

目的:建立牛黄消炎片中5种蒽醌类衍生物（芦荟大黄素、大黄酸、大黄素、大黄酚和大黄素甲醚）的含量测定方法。方法:采用高效液相色谱法

色谱柱为ZORBAX SB-C18色谱柱（4.6 mm×150 mm

5μm）

流动相乙腈-0.1%磷酸水溶液

梯度洗脱;流速1.0 mL·min-1;检测波长为226 nm;柱温40℃;进样量5μL。结果:芦荟大黄素、大黄酸、大黄素、大黄酚和大黄素甲醚5种成分在50 min内基线分离。峰面积（Y）对进样量（X）的标准曲线分别为芦荟大黄素Y=6 754.2X+2.078 9

r=0.999 9;大黄酸Y=4 191.1X-1.566 2

r=1.000 0;大黄素Y=5 029.4X-0.792 9

r=0.999 9;大黄酚Y=4 558.6X-2.863 7

r=1.000 0;大黄素甲醚Y=3 105.0X+114.41

r=0.990 7。加样回收率分别为（n=6）:芦荟大黄素100.8%（RSD 1.8%）;大黄酸100.3%（RSD 1.4%）;大黄素100.6%（RSD 1.2%）;大黄酚103.2%（RSD 2.4%）;大黄素甲醚100.5%（RSD 0.9%）。结论:采用高效液相色谱法同时测定牛黄消炎片中芦荟大黄素、大黄酸、大黄素、大黄酚和大黄素甲醚的含量

样品处理简便

测定结果准确

可用于该制剂的质量控制。

Abstract

Objective:To develop a method for the determination of five anthraquinone derivatives（aloeemodin

rhein

emodin

chrysophanol and physcion） in Niuhuang Xiaoyan tablets.Method:High performance liquid chromatography was performed on an ZORBAX SB-C18column（4.6 mm × 250 mm

5 μm） with acetonitrile-0.1% phosphoric acid as a mobile phase by gradient elution.The flow rate was 1.0 mL.min-1;the detection wavelength was set at 226 nm;the temperature of column was at 40 ℃;the injection volume was 5 μL.Result:Five compounds were separated at baseline within 50 min with good linearity.The regression equations were Y=6 754.2X +2.078 9

r=0.999 9（for aloe-emodin）

Y=4 191.1X-1.566 2

r=1.000 0（for rhein）

Y=5 029.4X-0.7929

r=0.999 9（for emodin）

Y=4 558.6X-2.863 7

r=1.000 0（for chrysophanol）

and Y=3 105.0X +114.41

r=0.990 7（for physcion）.The average recoveries（n=6） were:aloe-emodin 100.8%（RSD 1.8%）

rhein 100.3%（RSD 1.4%）

emodin 100.6%（RSD 1.2%）

chrysophanol 103.2%（RSD 2.4%） and physcion 100.5%（RSD 0.9%）.Conclusion:HPLC is a simple

accurate

stable

and reliable method in determining the content of aloe-emodin

rhein

emodin

chrysophanol and physcion in Niuhuang Xiaoyan tablets

and it can be used for quality control of this preparation.

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Related Institution

College of Pharmacy,Dali University,Dali

Institute of Chinese Materia Medica,Chongqing Three Gorges Medical College

Nanjing University of Chinese Medicine

Jiangsu Key Laboratory for Traditional Chinese Medicine Formulae Research

The Institute of Basic Theory,China Academy of Chinese Medical Sciences

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