Objective:To investigate the mechanisms of Weichangshu in promoting gastrointestinal motility.Method:Gastrointestinal smooth muscle cells were primarily cultured with tissues.Animals were divided into a lowdose of Weichangshu group（LW）

a moderate-dose of Weichangshu group（MW）

a high-dose of Weichangshu group（HW）

a cisapride group（CI） and a normal control group（NC）.The gastrointestinal smooth muscle cells were interfered with tested reagents for 24 h in different groups（normal group with DMEM for Group NC）)

and with 3-Amino

4-aminomethy1-2′

7′-difluorescein

diacetate（DAF-FMDA） for 30min for reaction

and release of NO was detected by using a Flow cytometry.Result:Compared with Group NC

the fluorescence intensities of NO release were significantly reduced in Group HW and Group MW（P < 0.01

P < 0.05）

which were similar to Group CI.Conclusion: Inhibition of generation of NO in gastrointestinal smooth muscle cells followed by inhibition of activation of correlated enzymes to antagonize formation of cGMP

leading to increase of intracellular Ca2 + to trigger series of physiological functions to accelerate gastrointestinal smooth muscle contraction may be involved in the mechanisms of Weichangshu for promoting gastrointestinal motility.