Effects of Modified Danggui Shaoyao Powder on the Expression of Inflammatory Cytokines and Phosphorylated MAPK Signal Molecules in Hippocampus Induced by Fibrillar Aβ<sub>42</sub>

HUANG De-hong1; LIU Meng-yuan2; YAN Xiao-feng2

doi:10.13422/j.cnki.syfjx.2011.02.043

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Effects of Modified Danggui Shaoyao Powder on the Expression of Inflammatory Cytokines and Phosphorylated MAPK Signal Molecules in Hippocampus Induced by Fibrillar Aβ₄₂

更新时间：2024-09-23

- Effects of Modified Danggui Shaoyao Powder on the Expression of Inflammatory Cytokines and Phosphorylated MAPK Signal Molecules in Hippocampus Induced by Fibrillar Aβ₄₂
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 2, Pages: 143-148(2011)
- 作者机构：
  
  1. 广州市中医医院
  2. 广州市中医中药研究所
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2011.02.043
  CLC： R285.5
- Published：2011
- 稿件说明：
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[1]黄德弘,刘孟渊,闫小峰.加味当归芍药散对纤丝状Aβ_(42)诱导脑内炎症因子及磷酸化MAPK信号分子表达的影响[J].中国实验方剂学杂志,2011,17(02):143-148.

HUANG De-hong1, LIU Meng-yuan2, YAN Xiao-feng2. Effects of Modified Danggui Shaoyao Powder on the Expression of Inflammatory Cytokines and Phosphorylated MAPK Signal Molecules in Hippocampus Induced by Fibrillar Aβ₄₂[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(2): 143-148.
[1]黄德弘,刘孟渊,闫小峰.加味当归芍药散对纤丝状Aβ_(42)诱导脑内炎症因子及磷酸化MAPK信号分子表达的影响[J].中国实验方剂学杂志,2011,17(02):143-148. DOI： 10.13422/j.cnki.syfjx.2011.02.043.

HUANG De-hong1, LIU Meng-yuan2, YAN Xiao-feng2. Effects of Modified Danggui Shaoyao Powder on the Expression of Inflammatory Cytokines and Phosphorylated MAPK Signal Molecules in Hippocampus Induced by Fibrillar Aβ₄₂[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(2): 143-148. DOI： 10.13422/j.cnki.syfjx.2011.02.043.

摘要

目的:观察Aβ42沉积对大脑海马炎症因子和磷酸化丝裂原活化蛋白激酶（mitogen activated protein kinase

MAPK）信号分子表达的影响及加味当归芍药散（JWDSS）的干预作用。方法:选用12周龄雌性SD大鼠

随机分为6组

应用立体定位注射技术对SD大鼠1次性大脑海马定位注射纤丝状Aβ42复制AD病理模型

并给予JWDSS（剂量分别为70.654

35.327 g.kg-1.d-1）进行干预

以西乐葆（剂量0.183 g.kg-1.d-1）为对照

用免疫组化（SABC）方法显示炎症因子及磷酸化MAPK信号分子表达情况

并用分析软件进行图像分析。结果:与正常对照组相比

AD模型组的白细胞介素-1β（interleukin-1β

IL-1β）

IL-6

phospho-p38（p-p38）

p-JNK

p-MEK3/6的阳性细胞染色面积均增加而染色强度值均下降（蛋白表达量与染色强度值成反比）;各给药组的IL-1β

IL-6阳性细胞染色面积较AD模型组下降;JWDSS高、低剂量组的IL-6及JWDSS高剂量组的IL-1β染色强度值均较AD模型组增加

而JWDSS低剂量组的IL-1β染色强度值亦呈增加的趋势;西乐葆对照组的IL-1β染色强度值亦较AD模型组增加

而IL-6染色强度值呈增加的趋势。另外

除了JWDSS低剂量组的p-JNK外

其余各给药组的p-p38

p-JNK

p-MEK3/6阳性细胞染色面积与AD模型组相比均明显下降

而染色强度值均较AD模型组明显增加。结论:Aβ42沉积可诱导大脑海马炎症反应和炎症细胞因子IL-1β

IL-6及p-p38

p-JNK

p-MEK3/6的过表达

而抑制炎症因子及磷酸化MAPK信号分子的过表达

从而抑制AD模型脑内炎症

可能是JWDSS拮抗AD的主要作用机制。

Abstract

Objective: To observe the effects of Aβ42 deposition on the expression of hippocampal inflammatory cytokines and phosphorylated mitogen-activated protein kinase（MAPK） signal molecules

and the intervention by Modified Danggui Shaoyao Powder（JWDSS）.Method: Alzheimer’s disease（AD） model was established by stereotactic injection of fibrillar Aβ42 into the hippocampus of 12 week-old female SD rats being divided into 6 groups.JWDSS（70.654

35.327 g·kg-1·d-1） was given for intervention

and celebrex（0.183 g·kg-1·d-1） was used as control.Expression of inflammatory cytokines and phosphorylated MAPK pathway signaling molecules were observed by immunohistochemistry（SABC） methods and SABC images were analyzed by an image analyzing software.Result: Compared with the control group

the positive stained areas of interleukin-1β（IL-1β）

IL-6 and phospho-p38（p-p38）

p-JNK and p-MEK3 /6 increased with decreased staining intensity in the modelgroup.Protein expression quantity was inversely proportional to the staining intensity.The positive stained areas of IL-1β

IL-6 and p-p38

p-JNK and p-MEK3 /6 in the treatment groups decreased and their staining intensity increased

compared with the AD model group.Conclusion:Aβ42 deposition in hippocampus can induce inflammation of the brain and over-expression of IL-1β

IL-6 and p-p38

p-JNK

p-MEK3 /6.Inhibiting the over-expression of inflammatory cytokines and phosphorylated MAPK signaling molecules may be a major antagonistic mechanism of JWDSS for AD.

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Related Author

HUANG De-hong

LIU Meng-yuan

YAN Xiao-feng

SUN Yuchen

ZHANG Yan

YIN Wenhao

ZHAO Shujun

ZHANG Muchen

Related Institution

Liaoning University of Traditional Chinese Medicine（TCM）

The Affiliated Hospital of Liaoning University of TCM

Henan Province Hospital of Traditional Chinese Medicine （Second Affiliated Hospital of Henan University of Chinese Medicine）

The Second Clinical Medical School of Henan University of Chinese Medicine

Zhengzhou Yihe Hospital

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Effects of Modified Danggui Shaoyao Powder on the Expression of Inflammatory Cytokines and Phosphorylated MAPK Signal Molecules in Hippocampus Induced by Fibrillar Aβ42

DOI：10.13422/j.cnki.syfjx.2011.02.043

摘要

Abstract

关键词

Keywords

references

Effects of Modified Danggui Shaoyao Powder on the Expression of Inflammatory Cytokines and Phosphorylated MAPK Signal Molecules in Hippocampus Induced by Fibrillar Aβ₄₂