Anti-hypoxia Effect of Hydrolysates Come from Enzymatic Process of Meat of Reared Crocodylus siamensis

ZHANG Shuo-feng1; LI Yao-wu2; ZHOU Man1; WANG Yu-rong1; SUN Jian-ning1

doi:10.13422/j.cnki.syfjx.2011.02.048

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Anti-hypoxia Effect of Hydrolysates Come from Enzymatic Process of Meat of Reared Crocodylus siamensis

更新时间：2024-09-23

- Anti-hypoxia Effect of Hydrolysates Come from Enzymatic Process of Meat of Reared Crocodylus siamensis
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 2, Pages: 166-168(2011)
- 作者机构：
  
  1. 北京中医药大学
  2. 北京洪源澳达生物技术发展有限公司
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2011.02.048
  CLC： R285.5
- Published：2011
- 稿件说明：
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[1]张硕峰,李耀武,周曼,王玉蓉,孙建宁.人工养殖暹罗鳄鱼肉酶解物提高缺氧耐受力的药效学验证[J].中国实验方剂学杂志,2011,17(02):166-168.

ZHANG Shuo-feng1, LI Yao-wu2, ZHOU Man1, et al. Anti-hypoxia Effect of Hydrolysates Come from Enzymatic Process of Meat of Reared Crocodylus siamensis[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(2): 166-168.
[1]张硕峰,李耀武,周曼,王玉蓉,孙建宁.人工养殖暹罗鳄鱼肉酶解物提高缺氧耐受力的药效学验证[J].中国实验方剂学杂志,2011,17(02):166-168. DOI： 10.13422/j.cnki.syfjx.2011.02.048.

ZHANG Shuo-feng1, LI Yao-wu2, ZHOU Man1, et al. Anti-hypoxia Effect of Hydrolysates Come from Enzymatic Process of Meat of Reared Crocodylus siamensis[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(2): 166-168. DOI： 10.13422/j.cnki.syfjx.2011.02.048.

摘要

目的:验证鳄鱼肉酶解物具耐缺氧作用。方法:以茚三酮-紫外分光光度法测定不同酶（胃蛋白酶、胰蛋白酶、木瓜酶、中性蛋白酶和木瓜酶+中性蛋白酶）的鳄鱼肉酶解产物的蛋白含量

并观察其酶解物提高小鼠的耐缺氧能力。结果:鳄鱼肉胃蛋白酶酶解产物的蛋白含量最高

鳄鱼肉胃蛋白酶酶解产物0.55

1.10 g.kg-1连续ig 20 d

可明显延长小鼠常压耐缺氧时间（与对照组比较P<0.05）。结论:鳄鱼肉胃蛋白酶酶解物具有提高缺氧耐受力的作用。

Abstract

Objective:To validate the anti-hypoxia activity in mice for hydrolysates come from the enzymatic process of meat of reared Crocodylus siamensis.Method:The protein content of five kinds of hydrolysates one from enzymolysis of the meat of reared crocodylus by pepsin

trypsin

papain

neutral protease and neutral protease plus papain separately was measured.The effect of anti-hypoxia of the products was observed in mice.Result:The protein content of hydrolysate come from enzymolysis of the meat of reared crocodylus by pepsin is the most.And hydrolysate of pepsin 0.55

1.10 g·kg-1 could extend survival time（there is significant difference between treatment group and control group

P<0.05） after intragastric administration of the hydrolysates once a day for 20 days.Conclusion: Hydrolysate of pepsin has effect of anti-hypoxia.

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