Objective: To observe the chronic toxicity damage of the refined products of bupleurum saikosaponin with alcohol elution to rats who were continuously administrated for 45 days and its recovery condition.Method:Separately lavage different dose refined products of Bupleurum saikosaponin with alcohol elution to rats

and the high

middle and low lose-group are separately 60

30

15 mg·kg-1 caculated by total saikosaponin

the general conditions were observed and the related indexes were detected

such as the routine control of blood

hepatic function

renal function

the metabolism condition of lipids and the glycometabolism indexes;the rats were dissected and precisionly weigh the weight of heart

liver

spleen

lung

renal

and calculated the ratio of organ to body;thehistopathologic examination of hepatic tissue were examined.The remaining rats were stopped administrating

and recovered for 30 days

observed the recovery condition of rats

and the above indexes were detected.Result:Different dose refined products of Bupleurum saikosaponin with alcohol elution could induce the rats’ body weight decreasing

the level of alanine aminotransterase（ALT）

aspartate aminotransterase（AST）

total bilirubin（TBI）

total protein（TPC）

and blood urea nitrogen（BUN） in serum obviously increasing

alburnin（ALB） in serum obviously decreasing

the liver weight and the ratio of liver to body increasing

and the hepatic tissue damage was obvious in the histopathologic examination of hepatic tissue;the above-mentioned change gradually aggravated with dose increasing

and it was the obvious discrepancy compared with control group.The influence on the content of alkaline phosphatase（AKP）

glucose（Glu）

cholesterol（CHO） and Cr was not obvious.The above indexes of recovered rats had no obvious discrepancy compared with control group.Conclusion:The long term and certain dose administration of refined products of Bupleurum saikosaponin with alcohol elution to rats could induce obvious cumulative toxicity

and the mainly toxical injury sites were liver and renal

after the 30 d recovery period observation

the toxicity was basically recovered.