HPLC-DAD-ELSD Fingerprint of Ziziphi Spinosae Semen from Different Sources

YAN Yan; DU Chen-hui; PEI Xiang-ping; ZHAO Fei-fei; YUAN Hong-xia; ZHANG Shuo-sheng

doi:10.13422/j.cnki.syfjx.2011.04.026

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HPLC-DAD-ELSD Fingerprint of Ziziphi Spinosae Semen from Different Sources

更新时间：2024-09-23

- HPLC-DAD-ELSD Fingerprint of Ziziphi Spinosae Semen from Different Sources
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 4, Pages: 67-69(2011)
- 作者机构：
  
  山西中医学院中药系
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2011.04.026
  CLC： R284.1
- Published：2011
- 稿件说明：
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[1]闫艳,杜晨晖,裴香萍,赵飞飞,原红霞,张硕生.不同产地酸枣仁药材HPLC-DAD-ELSD指纹图谱[J].中国实验方剂学杂志,2011,17(04):67-69.

YAN Yan, DU Chen-hui, PEI Xiang-ping, et al. HPLC-DAD-ELSD Fingerprint of Ziziphi Spinosae Semen from Different Sources[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(4): 67-69.
[1]闫艳,杜晨晖,裴香萍,赵飞飞,原红霞,张硕生.不同产地酸枣仁药材HPLC-DAD-ELSD指纹图谱[J].中国实验方剂学杂志,2011,17(04):67-69. DOI： 10.13422/j.cnki.syfjx.2011.04.026.

YAN Yan, DU Chen-hui, PEI Xiang-ping, et al. HPLC-DAD-ELSD Fingerprint of Ziziphi Spinosae Semen from Different Sources[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(4): 67-69. DOI： 10.13422/j.cnki.syfjx.2011.04.026.

摘要

目的:建立不同产地酸枣仁药材HPLC-DAD-ELSD指纹图谱

为科学评价和有效控制其质量提供可靠的方法。方法:采用HPLC-DAD-ELSD方法测定了14个产地酸枣仁药材样品

采用药典委员会颁布的中药色谱指纹图谱相似度评价系统2004A进行评价

建立了共有模式

以相关度评价图谱的相似性。结果:14个产地酸枣仁药材的色谱指纹图谱黄酮类有15个共有峰

皂苷类有11个共有峰。建立的方法对酸枣仁药材中黄酮类成分、皂苷类成分均有良好的分离

一次进样同时测定了两类不同成分的指纹图谱。结论:本方法可用于酸枣仁药材中黄酮类成分和皂苷类成分指纹图谱的测定

为控制酸枣仁药材的质量提供依据。

Abstract

Objective:To establish the HPLC-DAD-ELSD fingerprint of Ziziphi Spinosae Semen grown indif-ferent provinces and provide new methods for scientific evaluation and effectively controlling the quality of Semen Ziziphi Spinosae.Method:Fourteen Semen Ziziphi Spinosae samples from different provinces were determined by u-sing HPLC-DAD-ELSD technique.The software"similarity evaluation system for chromatographic fingerprint of Chi-nese material medical"was applied to analyze date

and established the mutual model.The similarity of chromatogram was evaluated with correlation degree.Result:There were fifteen common peaks of flavonoids and eleven common peaks of saponins in the fingerprint of Semen Ziziphi Spinosae.The established method is good for the separation of flavonoids and saponins from Semen Ziziphi Spinosae and simultaneous determination of the two differentcomponents in one sample injection.Conclusion:he method is workable to simultaneously determine flavonoids and saponins fin-gerprint from semen Ziziphi Spinosae

and provide experimental data for comprehensive evaluation of the quality of Semen Ziziphi Spinosae.

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Related Author

YAN Yan

DU Chen-hui

PEI Xiang-ping

ZHAO Fei-fei

YUAN Hong-xia

ZHANG Shuo-sheng

CHEN Aijun

HUANG Siqi

Related Institution

Hunan Polytechnic of Environment and Biology

School of Pharmacy，Hunan Provincial Key Laboratory of Drugability and Preparation Modification of Traditional Chinese Medicine，Hunan University of Chinese Medicine

Affiliated Hospital of Hunan Academy of Chinese Medicine

Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization， Nanjing University of Chinese Medicine

Jiangsu Health Vocational College

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