Determination of Three Flavonoids in Lespedeza virgata by RP-HPLC

HUANG Zhi-qin; CHENG Qi-lai; LI Hong-liang

doi:10.13422/j.cnki.syfjx.2011.04.027

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Determination of Three Flavonoids in Lespedeza virgata by RP-HPLC

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- Determination of Three Flavonoids in Lespedeza virgata by RP-HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 4, Pages: 70-72(2011)
- 作者机构：
  
  赣南医学院药学院
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2011.04.027
  CLC： R284.1
- Published：2011
- 稿件说明：
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[1]黄志勤,程齐来,李洪亮.RP-HPLC测定细梗胡枝子中3种黄酮类有效成分[J].中国实验方剂学杂志,2011,17(04):70-72.

HUANG Zhi-qin, CHENG Qi-lai, LI Hong-liang. Determination of Three Flavonoids in Lespedeza virgata by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(4): 70-72.
[1]黄志勤,程齐来,李洪亮.RP-HPLC测定细梗胡枝子中3种黄酮类有效成分[J].中国实验方剂学杂志,2011,17(04):70-72. DOI： 10.13422/j.cnki.syfjx.2011.04.027.

HUANG Zhi-qin, CHENG Qi-lai, LI Hong-liang. Determination of Three Flavonoids in Lespedeza virgata by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(4): 70-72. DOI： 10.13422/j.cnki.syfjx.2011.04.027.

摘要

目的:建立一种高效液相色谱测定细梗胡枝子中槲皮素、芦丁、山柰酚含量的方法。方法:采用反相高效液相色谱法

Agilent 1100 ZORBAX Eclipse XDB-C18色谱柱（4.6 mm×150 mm

5μm）

二极管阵列检测器（DAD）

甲醇-0.2%磷酸溶液（60∶40）为流动相

检测波长251 nm

流速1.0 mL.min-1

柱温35℃。结果:槲皮素在0.210～4.19μg

芦丁在0.084～1.68μg

山奈酚在0.066～1.33μg

峰面积与进样量呈良好的线性关系;芦丁、槲皮素和山奈酚的加样回收率分别为99.48%

98.53%

99.33%

RSD分别为0.51%

1.35%

2.13%（n=5）。结论:该方法精密度高、重复性和分离效果较好

分析快速准确

适合于该药材及提取物中槲皮素、芦丁和山奈酚3种黄酮类含量的测定

可作为该药材质量控制的参考依据。

Abstract

Objective:To establish a quantification method for determination of quercetin

rutoside and kaempferol in Lespedeza virgata by HPLC.Method:RP-HPLC was applied on the determination of quercetin

rutoside and kaempferol in L.virgata with C18 column（4.6 mm × 250 mm

5 μm）

DAD detector

and a mixture of acetonitrile-0.2% phosphoric acid（60∶ 40） as a mobile phase with the flow rate at 1.0 mL.min-1.Three flavonoids were detected at 251 nm

column temperature was set at 35 ℃.Result:Quercetin

rutoside and kaempferol were linear in the range of 0.210-4.19

0.084-1.68

0.066-1.33 μg.The average recovery of rutoside

quercetin and kaempferol was 99.48%

98.53% and 99.33% with RSD（n = 5） 0.51%

1.35% and 2.13% respectively.Conclution:The method is accurate and sensitive

which can be used rapidly for the content determinatinon of rutoside

quercetin and kaempferol from L.virgata.It can be applied to the quality control of this crude drug.

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