Comparison of Protein Extraction Methods of Trichosanthis Fructus

SONG Yang-mei; LI Feng; ZHOU Li-man

doi:10.13422/j.cnki.syfjx.2011.04.035

您当前的位置：

首页 >

文章列表页 >

Comparison of Protein Extraction Methods of Trichosanthis Fructus

更新时间：2024-09-23

- Comparison of Protein Extraction Methods of Trichosanthis Fructus
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 4, Pages: 95-97(2011)
- 作者机构：
  
  山东中医药大学药学院
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2011.04.035
  CLC： R284.2
- Published：2011
- 稿件说明：
移动端阅览
[1]宋艳梅,李峰,周丽曼.瓜蒌种子蛋白质6种提取方法比较[J].中国实验方剂学杂志,2011,17(04):95-97.

SONG Yang-mei, LI Feng, ZHOU Li-man. Comparison of Protein Extraction Methods of Trichosanthis Fructus[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(4): 95-97.
[1]宋艳梅,李峰,周丽曼.瓜蒌种子蛋白质6种提取方法比较[J].中国实验方剂学杂志,2011,17(04):95-97. DOI： 10.13422/j.cnki.syfjx.2011.04.035.

SONG Yang-mei, LI Feng, ZHOU Li-man. Comparison of Protein Extraction Methods of Trichosanthis Fructus[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(4): 95-97. DOI： 10.13422/j.cnki.syfjx.2011.04.035.

摘要

目的:建立适合于瓜蒌种子的蛋白质提取方法。方法:比较水溶液直接提取法（3种）、三氯乙酸（TCA）-丙酮法、三羟甲基氨基甲烷（Tris）-丙酮法、蛋白质提取液提取法等6种蛋白质提取方法所获瓜蒌种子总蛋白含量、聚丙烯酰胺凝胶电泳的谱带等方面的差异。结果:蛋白质提取液提取法、TCA-丙酮法提取蛋白含量较高

但杂质多

电泳谱带严重展宽而且明显拖尾;Tris-丙酮法提出的蛋白质种类缺失严重;直接提取法提取蛋白含量少

但杂质少

电泳谱带清晰

离心2次谱带更加清晰。结论:水溶液直接提取法最适合用于聚丙烯酰胺凝胶电泳

蛋白质提取液提取法提出蛋白质含量最多。

Abstract

Objective:To determine the protein extraction method of Fructus Trichosanthis.Method:The protein yield

protein purity and polyacrylamide gel electrophoresis（PAGE） patterns were determined and analyzed.Result: More proteins were extracted by the method of protein extracted liquid and trichloroacetic acid（TCA）-ace-tone

but their bands were not clear and the resolution was low because of more impurities extracted.It waseasy to produce high-abundance proteins and interfered in the electrophoresis resolution.Protein yield and band numbers by Tris-base /acetone were the lowest.Besides

the protein content by commonly used direct extraction was low

but with less impurity

clearer bands and highest resolution.Conclusion: Direct extraction is the most suitable method for PAGE and more protein can be extracted by protein extracted liquid extraction method.

关键词

Keywords

references

Views

674

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Comparison of Protein Extraction Methods of Trichosanthis Fructus

Discrimination of Rana dybowskii, Its Analogues and Counterfeits Based on Polyacrylamide Gel Electrophoretograms Combined with Cluster Analysis and Multivariate Statistical Analysis

Comparative Study of the Body and Tail Protein of from Different Habitats

Determination of Total Sugar and Reducing Sugar in Processed Fructus Trichosanthis

Radix Aconiti Compatibility of Fructus Trichosanthis on Experimental Study of Long-term Toxicity in Rats

Related Author

ZHOU Li-man

LI Feng

SONG Yang-mei

Meng LAN

Hui YAO

Jing-feng LI

Xue-feng BIAN

Jing-wei LYU

Related Institution

Jilin Ginseng Academy in Changchun University of Chinese Medicine

Jilin Xinshui Science and Technology Development Co. Ltd.

Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College

Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰