Objective:To observe the effect of the berberine（BBR）on ischemia-reperfusion for renal tubu-lar epithelial cells（NRK-52E）of rat.Method: Using chemical hypoxia cobalt chloride（CoCl2） to stimulate NRK-52E cells to develop a chemical model of hypoxia-reoxygenation injury.Then

the cells were treated with different concentrations（1 × 10-6

1 × 10-5

1 × 10-4 mol.L-1）of BBR.Methylthiazolyldiphenyl-tetrazolium bromide（MTT） was used to test the activity of the cells.Malondialdehyde（MDA）and superoxide dismutase（SOD） content of each group were determined;Hoechst33258 staining was used to detect the morphological changes of apoptotic cells of each group;Annexin V-FITC /PI staining was applied to detect the rate of apoptosis cell in each group.Result: MTT results showed that 1 × 10-4 mol.L-1 BBR was toxic to the cells

but 1 × 10-6 mol.L-1and 1 × 10-5 mol.L-1BBR could increase the ischemia-reperfusion group cell survival

especially in 1 × 10-5 mol.L-1 for the high;Ho-echst33258 showed almost no apoptotic cells in the normal group

in the model group there were mostly nuclear stai-ning of apoptotic cells

while BBR（1 × 10-5 mol.L-1） pretreatment significantly reduced apoptosis cells;With the normal control groups

the MDA of ischemia-reperfusion group content was significantly higher（P < 0.05）

but the SOD was significantly lower（P < 0.05）.The pretreatment with BBR group

MDA was significantly lower than that of before treatment（P < 0.05）

SOD was higher than the ischemia-reperfusion group（P < 0.05）

the role of BBR depends on the concentration between 1 × 10-6 mol.L-1 and 1 × 10-5 mol.L-1.The Flow Cytometry showed that the apoptosis rate of 1 × 10-5

1 × 10-6 mol.L-1 BBR group and ischemia-reperfusion group were 11.1%

32.2% and 56.2%

and the differences were significantly（P < 0.05）.Conclusion: The pretreatment with BBR on the ischemi-a-reperfusion cell has protective effects on renal tubular epithelial cells

especially the concentration of 1 × 10-5 mol.L-1 was most effective.