Objective:To investigate pharmacokinetics process of the major components of the extract Corydalis tetrahydropalmatine（tetrahydropalmatine

THP） and d-corydaline（corydaline

CDL） in rats.Method:U-sing LC-MS /MS to measure plasma concentration at different time after ig administration concentration of Corydalis extracts was determined

WinNonlin software was used to calculate pharmacokinetic parameters.Result:ESI（+）i-ons under the condition of using MRM mode with m /z370-354

m /z 356-340 was used to detecte CDL

THP

while the m /z 306-140 was used to detect the internal standard anisodamine（anisodaminehydrochloride

ADM）.In the range of 1.13-565

1.5-750 ng.mL-1

CDL

THP showed good linearity

high

medium and low recovery rates of THP

and CDL

day and inter-day precision met the demands of quantification

Cmax were（84.3 ± 9.2）

（157.3 ± 99.6）

（497.1 ± 71.3）ng.mL-1 and（30.8 ± 1.3）

（54.4 ± 22.7）

（91.2 ± 15.4） ng.mL-1

Tmax were（54.8 ± 6.9）

（119.2 ± 5.7）

（62.5 ± 7.5） min and（31.5 ± 0.07）

（65.6 ± 10.6）

（111.5 ± 4.2）min.Conclusion: LC-MS /MS had good selectivity

high sensitivity for the determination of plasma sample.