Effective Composition Analysis of Two Kinds of Preparation Technology for Yinhuang Decoction

GUO Wu-yan; KONG Huan-yu; ZHU Jia; YANG Li-ping

doi:10.13422/j.cnki.syfjx.2011.05.034

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Effective Composition Analysis of Two Kinds of Preparation Technology for Yinhuang Decoction

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- Effective Composition Analysis of Two Kinds of Preparation Technology for Yinhuang Decoction
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 5, Pages: 8-11(2011)
- 作者机构：
  
  中国中医科学院骨伤科研究所
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2011.05.034
  CLC： R283.6
- Published：2011
- 稿件说明：
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[1]郭武艳,孔焕宇,朱嘉,杨丽平.不同制备工艺银黄方中有效成分分析[J].中国实验方剂学杂志,2011,17(05):8-11.

GUO Wu-yan, KONG Huan-yu, ZHU Jia, et al. Effective Composition Analysis of Two Kinds of Preparation Technology for Yinhuang Decoction[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(5): 8-11.
[1]郭武艳,孔焕宇,朱嘉,杨丽平.不同制备工艺银黄方中有效成分分析[J].中国实验方剂学杂志,2011,17(05):8-11. DOI： 10.13422/j.cnki.syfjx.2011.05.034.

GUO Wu-yan, KONG Huan-yu, ZHU Jia, et al. Effective Composition Analysis of Two Kinds of Preparation Technology for Yinhuang Decoction[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(5): 8-11. DOI： 10.13422/j.cnki.syfjx.2011.05.034.

摘要

目的:应用梯度洗脱高效液相色谱法

对2种制备工艺的银黄方中有效成分及含量进行分析比较。方法:采用DiamonsilC18（4.6 mm×250 mm

5μm）色谱柱

乙腈-0.1%磷酸溶液作流动相梯度洗脱

以流速1.0 mL·min-1

在检测波长276 nm和328 nm处测定银黄方中有效成分绿原酸、黄芩苷和汉黄芩苷的含量。结果:绿原酸在0.011 8～0.059μg线性关系良好（r=0.999 9）

平均回收率为98.30%;黄芩苷在0.19～9.5μg线性关系良好（r=0.999 9）

平均回收率为102.87%;汉黄芩苷在0.010 4～0.52μg线性关系良好（r=0.999 9）

平均回收率为104.33%。银黄方膜分离工艺得到3种成分固含量总和（>50%）高于银黄口服液（13.36%）。结论:本法可用于银黄方中上述3种成分的含量测定

膜分离工艺得到的有效部位含量占提取物的50%以上。

Abstract

Objective:To establish an HPLC method by gradient elution for comparative analysis of the ef-fective composition in two kinds of Yinhuang decoction.Method:The column was Diamonsil C18（4.6 mm × 250 mm

5 μm）.The mobile phase was consisted of acetonitrile-0.1% phosphoric acid with gradient elution.The flow rate was 1.0 mL·min-1 and the detection wavelength were at 276 nm and 328 nm for the content determination of chlorogenic acid

baicalin and wogonoside.Results:Chlorogenic acid

baicalin and wogonoside were linear in the range of 0.011 8-0.059 μg（r = 0.999 9）

0.19-9.5 μg（r = 0.999 9） and 0.0104-0.52 μg（r = 0.999 9） respec-tively.The average recoveries of them were 98.30%

102.87% and 104.33% respectively.The total solid content of Yinhuang decoction separated by filtration membrane was more than 50% compared with that of Yinhuang oral liq-uid（13.36%）.Conclusion:The HPLC can be applied to content determination of chlorogenic acid

baicalin and wogonoside in Yinhuang decoction

the content of effective parts separated by filtration membrane was more than 50%.Filtration membrane was conducive to developing the fifth class new drugs.

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