Separation and Purification of Quercetin-3-O-glucoside from Lophatherum gracile by High-speed Counter-current Chromatography Coupled with UNIFAC Mathematical Model

LIN Zhi-xin 1; GU Xue-zhu2; LIU Jun 1

doi:10.13422/j.cnki.syfjx.2011.05.037

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Separation and Purification of Quercetin-3-O-glucoside from Lophatherum gracile by High-speed Counter-current Chromatography Coupled with UNIFAC Mathematical Model

更新时间：2024-09-23

- Separation and Purification of Quercetin-3-O-glucoside from Lophatherum gracile by High-speed Counter-current Chromatography Coupled with UNIFAC Mathematical Model
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 5, Pages: 23-27(2011)
- 作者机构：
  
  1. 大连医科大学附属第一医院
  2. 中国中医科学院中药研究所
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2011.05.037
  CLC： R284
- Published：2011
- 稿件说明：
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[1]林治鑫,顾雪竹,刘珺.高速逆流色谱结合UNIFAC数学模型分离纯化淡竹叶中的槲皮素-3-O-葡萄糖苷[J].中国实验方剂学杂志,2011,17(05):23-27.

LIN Zhi-xin 1, GU Xue-zhu2, LIU Jun 1. Separation and Purification of Quercetin-3-O-glucoside from Lophatherum gracile by High-speed Counter-current Chromatography Coupled with UNIFAC Mathematical Model[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(5): 23-27.
[1]林治鑫,顾雪竹,刘珺.高速逆流色谱结合UNIFAC数学模型分离纯化淡竹叶中的槲皮素-3-O-葡萄糖苷[J].中国实验方剂学杂志,2011,17(05):23-27. DOI： 10.13422/j.cnki.syfjx.2011.05.037.

LIN Zhi-xin 1, GU Xue-zhu2, LIU Jun 1. Separation and Purification of Quercetin-3-O-glucoside from Lophatherum gracile by High-speed Counter-current Chromatography Coupled with UNIFAC Mathematical Model[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(5): 23-27. DOI： 10.13422/j.cnki.syfjx.2011.05.037.

摘要

目的:建立一个经济有效的方法用于淡竹叶Lophatherum gracile中槲皮素-3-O-葡萄糖苷的分离纯化。方法:采用高速逆流色谱（high-speed counter-current chromatography

HSCCC）进行分离纯化

所用溶剂体系为乙酸乙酯-正丁醇-水（2∶1∶3）

其上相和下相是根据UNIFAC数学模型单独配制

并与传统配制方法配制所得溶剂系统的分离效率进行比较;分离所得产物的纯度经HPLC检测

结构经MS

NMR鉴定。结果:HSCCC使用UNIFAC数学模型单独配制的溶剂系统成功的从竹叶中制备出纯度高于99%的槲皮素-3-O-葡萄糖苷

与传统溶剂系统配制方法相比

节省了乙酸乙酯、正丁醇和水的用量。结论:在淡竹叶中槲皮素-3-O-葡萄糖苷的HSCCC分离过程中

使用UNIFAC单独配制的溶剂系统既可保证较好分离效果

又可节省大量的有机溶剂

减少了资源浪费

益于环保。

Abstract

Objective:To develop an economic and sensitive method for separation of quercetin-3-O-gluco-side from Lophatherum gracile.Method:High-speed counter-current chromatography（HSCCC） and UNIFAC math-ematic model were successfully used to separate the target component.The solvent system composed of ethyl acetate-n-butanol-water（2:1:3） was singly prepared by UNIFAC mathematic model

and the comparison of the results pro-duced by HSCCC of using the solvent system prepared by UNIFAC and the conventional method were carried out.Result:The quercetin-3-O-glucoside with purity > 99% was successfully separated from L.gracile by HSCCC using the singled prepared solvent system

which had no difference compared with the classic method for preparation of the solvent system

but much of ethyl acetate

n-butanol and water were saved.Conclusion:In the HSCCC separation of quercetin-3-O-glucoside from L.gracile

using the single prepared solvent system by UNIFAC mathematic model can not only save resource

but also protect environment.

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Related Author

LIN Zhi-xin

GU Xue-zhu

LIU Jun

YU Wantong

HOU Kangxin

SU Xinyao

XUE Qiang

WANG Caixia

Related Institution

College of Food Science and Biology， Hebei University of Science and Technology

Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences

School of Pharmaceutical Sciences， Guangzhou University of Chinese Medicine

Guangdong Standardized Processing Engineering Technology Research Center of Traditional Chinese Medicine（TCM），School of TCM， Guangdong Pharmaceutical University

College of pharmacy,Beijing University of Chinese Medicine

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