Research on Improvement of Propolis Extracting Technology

SUN Jing1; XUE Rui3; LIU Jie-qiong1

doi:10.13422/j.cnki.syfjx.2011.05.039

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Research on Improvement of Propolis Extracting Technology

更新时间：2024-09-23

- Research on Improvement of Propolis Extracting Technology
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 5, Pages: 33-36(2011)
- 作者机构：
  
  1. 陕西中医学院药学院
  2. 湖南中医药大学
  3. 宁夏药品检验所
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2011.05.039
  CLC： R284
- Published：2011
- 稿件说明：
移动端阅览
[1]孙静,薛瑞,刘洁琼.蜂胶提取工艺的改进[J].中国实验方剂学杂志,2011,17(05):33-36.

SUN Jing1, XUE Rui3, LIU Jie-qiong1. Research on Improvement of Propolis Extracting Technology[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(5): 33-36.
[1]孙静,薛瑞,刘洁琼.蜂胶提取工艺的改进[J].中国实验方剂学杂志,2011,17(05):33-36. DOI： 10.13422/j.cnki.syfjx.2011.05.039.

SUN Jing1, XUE Rui3, LIU Jie-qiong1. Research on Improvement of Propolis Extracting Technology[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(5): 33-36. DOI： 10.13422/j.cnki.syfjx.2011.05.039.

摘要

目的:通过研究工艺改进前后蜂胶提取物总黄酮含量及出膏率的变化

得到改进提取工艺参数。方法:以总黄酮含量为考察指标

对温浸法、超声提取法、冷浸法等3种提取方法以及提取温度进行考察;以总黄酮含量和出膏率为考察指标

采用L934正交设计对乙醇体积分数、提取时间、乙醇用量、提取次数4个因素进行考察;比较蜂胶除蜡前后总黄酮含量和出膏率的变化。结果:蜂胶最佳提取工艺为10倍量75%乙醇温浸3次

每次2 h。蜂胶除蜡后提取

总黄酮含量增加14.43%

出膏率降低61.71%。结论:蜂胶除蜡后

不降低有效部位含量同时可大大降低出膏率

利于制剂

该方法优于传统直接提取有效部位工艺

且简便可行。

Abstract

Objective:To research the changes of flavonoids content and the rate of extractum extracted be-tween improvement technology and traditional technology

extraction technique to be improved.Method:Rutin was used as reference substance to determine the total flavonoid content in propolis by UV spectrophotometry.three dif-ferent extraction methods including soaking method

ultrasonic extraction

cold soak and the extraction temperature were investigated with total flavonoids and the rate of extractum extracted as the investigation indexes;4 factors of ethanol concentration

extraction time and ethanol

extraction times were investigated by orthogonal design.Parallel testing was adopted in the investigations.Result:Extraction times had significant impact on the total flavonoids of propolis at 60 ℃.The optimum technology conditions were as follows:10 times amount of 75% ethanol

soaking 3 times

extracted for 2 hours each time.The total flavonoids increased 14.43% and the rate of extractum extracted decreased 61.71%.Conclusion:Propolis wax removed before the effective parts of the extraction could greatly reduce the cream and did not decrease the effective site content

better than the effective parts of traditional direct extraction process and the method is simpler.

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