Determination of Content and Related Substances in Schizonepetin by HPLC

SUN Yi1; GENG Ting1; SHAN Ming-qiu1; ZHANG Li1; DING An-wei1

doi:10.13422/j.cnki.syfjx.2011.05.042

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Determination of Content and Related Substances in Schizonepetin by HPLC

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- Determination of Content and Related Substances in Schizonepetin by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 5, Pages: 52-55(2011)
- 作者机构：
  
  1. 南京中医药大学
  2. 江苏省方剂研究重点实验室
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2011.05.042
  CLC： R284.1
- Published：2011
- 稿件说明：
移动端阅览
[1]孙熠,耿婷,单鸣秋,张丽,丁安伟.HPLC测定荆芥内酯的含量和有关物质[J].中国实验方剂学杂志,2011,17(05):52-55.

SUN Yi1, GENG Ting1, SHAN Ming-qiu1, et al. Determination of Content and Related Substances in Schizonepetin by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(5): 52-55.
[1]孙熠,耿婷,单鸣秋,张丽,丁安伟.HPLC测定荆芥内酯的含量和有关物质[J].中国实验方剂学杂志,2011,17(05):52-55. DOI： 10.13422/j.cnki.syfjx.2011.05.042.

SUN Yi1, GENG Ting1, SHAN Ming-qiu1, et al. Determination of Content and Related Substances in Schizonepetin by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(5): 52-55. DOI： 10.13422/j.cnki.syfjx.2011.05.042.

摘要

目的:建立荆芥内酯的含量测定方法和有关物质的分析方法。方法:指定以Waters XTerra MS C18色谱柱进行有关物质分析

指定以Kromasil C18色谱柱进行含量测定

以甲醇-水（45∶55）为流动相（氢氧化钠调pH至10.0）

流速为0.8mL·min-1

柱温30℃

检测波长216 nm。结果:荆芥内酯与有关物质及各降解成分完全分离;荆芥内酯质量浓度在0.012 6～0.403 6 g·L-1与峰面积呈良好线性关系

r=0.999 9;最低检测限0.25μg

最小定量限为0.63μg;平均回收率100.04%

RSD为1.40%。结论:该方法操作简便、快速、准确

灵敏度高

可用于荆芥内酯含量及有关物质的测定。

Abstract

Objective:To establish an HPLC method for the determination of content and related substances in schizonepetin.Method:Two stability columns based on silica gel were used respectively（Waters XTerra MS C18 column for related substances analysis;And Kromasil C18 column for content determination）.The mobile phase consisted of methanol-water（45:55）

and when analyzing substances destroyed by alkali

the pH value of the mobile phase should be adjusted to 10.0 by sodium hydroxide.Both of the two columns were maintained at a constant temperature of about 30 ℃.The flow rate was 0.8 mL per minute.The UV detection wavelength was set at 216 nm.Result:Related substances and degraded substances were completely separated from schizonepetin.The linear range of determination was 0.012 6-0.403 6 g·L-1 with the correlation coefficient of 0.999 9.The LOD was 0.25 μg

and the LOQ was 0.63 μg.The average recovery was 100.04%

RSD 1.40%.Conclusion:The method is simple

rapid

accurate

and sensitive and it is suitable for the determination of the content and related substances in schizonepetin.

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