ZHU Gen-hua1, WAN Yan-ting2, LU Ke3, et al. Content Determination of Betulinic Acid in Rosae Laevigata Michx by HPLC-MS/MS[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(5): 55-57.
ZHU Gen-hua1, WAN Yan-ting2, LU Ke3, et al. Content Determination of Betulinic Acid in Rosae Laevigata Michx by HPLC-MS/MS[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(5): 55-57. DOI: 10.13422/j.cnki.syfjx.2011.05.043.
Content Determination of Betulinic Acid in Rosae Laevigata Michx by HPLC-MS/MS
Objective:To establish an HPLC-MS/MS method for determination of the content of Betulinic Acid in Rosae Laevigata michx.Method:Chromatograthy was performed on a 3 μm Diamonsil C18(2) column with the column temperature at 30 ℃.and the sample size at 10 μL.The mobile phase composition was methanol-water(0.2% formic acid)(84:16).The flow rate was 0.30 mL·min-1.Result:The betulinic acid had a good linearity re-lationship(r = 0.999 3
n = 6) between 4.8-230.4 ng.mL-1.The average recovery of betulinic acid in Rosae laevi-gata michx was 98.3%(RSD 2.6%).Conclusion:This method is simple