ZHOU Nong, DUAN Bao-zhong, XIA Cong-long, et al. Research on Changing Regulation of Ingredients in Paris polyphylla var. yunnanensis at Different Growth[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(5): 70-73.
ZHOU Nong, DUAN Bao-zhong, XIA Cong-long, et al. Research on Changing Regulation of Ingredients in Paris polyphylla var. yunnanensis at Different Growth[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(5): 70-73. DOI: 10.13422/j.cnki.syfjx.2011.05.046.
Research on Changing Regulation of Ingredients in Paris polyphylla var. yunnanensis at Different Growth
摘要
目的:建立滇重楼HPLC指纹图谱
考察滇重楼不同生长期主要化学成分的变化。方法:采用高效液相色谱法。色谱柱为Agela Venusil XBP-C18柱(4.6 mm×250 mm
Objective:To establish HPLC chromatographic fingerprint of Paris polyphylla var.yunnanensis and investigate variation of the main components in samples at various collecting seasons.Method:The main com-ponents were separated on a Agela Venusil XBP-C18(4.6 mm × 250 mm
5 μm) column and detected at 210 nm by gradient elution at the flow rate of 1.0 mL·min-1 using acetonitrile-water as the mobile phase.Column temperature was at 35 ℃.Result:A satisfactory method for HPLC fingerprint determination of P.polyphylla var.yunnanensis was obtained.The established fingerprint showed that 20 peaks were common and 4 peaks were identified.The main com-ponents of P.polyphylla var.yunnanensis differed from collecting seasons.Conclusion:The method can be used for quality control and provide a valuable reference for Good Agriculture Practice of P.polyphylla var.yunnanensis.
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Related Author
ZHOU Nong
DUAN Bao-zhong
XIA Cong-long
XU Ping
YANG Xian-hao
ZHENG Xiao-ke
DONG San-li
FENG Wei-sheng
Related Institution
Hunan Polytechnic of Environment and Biology
School of Pharmacy,Hunan Provincial Key Laboratory of Drugability and Preparation Modification of Traditional Chinese Medicine,Hunan University of Chinese Medicine
Affiliated Hospital of Hunan Academy of Chinese Medicine
Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine