LIU Jing-shu, QIAO Wei, HAO Lan-fang, et al. Identification and Determination of Jujuboside A in Suanzaoren Hehuan Formula[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(5): 77-79.
LIU Jing-shu, QIAO Wei, HAO Lan-fang, et al. Identification and Determination of Jujuboside A in Suanzaoren Hehuan Formula[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(5): 77-79. DOI: 10.13422/j.cnki.syfjx.2011.05.048.
Identification and Determination of Jujuboside A in Suanzaoren Hehuan Formula
摘要
目的:建立中药复方酸枣仁合欢方中酸枣仁皂苷A的定性鉴别以及高效液相色谱-蒸发光散射检测法的含量测定方法。方法:采用薄层色谱法对方剂中的有效成分酸枣仁皂苷A进行鉴别。采用Ameritech Accurasil C18色谱柱(4.6 mm×250 mm
5μm)
流动相为甲醇-水(60∶40)
流速1.0 mL·min-1
柱温35℃
检测器漂移管温度102℃。结果:在薄层色谱图谱中可以检出酸枣仁皂苷A
斑点清晰可见
重复性好。酸枣仁皂苷A检测浓度在0.6~3.0μg与峰面积呈良好的线形关系(r=0.999 9)
平均回收率为100.16%(RSD 1.63%)。结论:定性定量方法简便、准确、重复性好
可作为酸枣仁合欢方的质量控制方法。
Abstract
Objective:To establish a TLC method for identification of Semen Zizyphi Spinosae and a HPLC-ELSD method for determination of jujuboside A in Suanzaoren Hehuan Formula.Method:Quality of Semen Zizyphi Spinosae was identified with thin layer chromatography.The analytical column was Ameritech Accurasil-ODS(4.6 mm × 250 mm
5 μm).The mobile phrase consist of methanol-water(60:40).The flow rate was 1.0 mL·min-1.The column temperature was set at 35 ℃.The tube temperature of the detector was 102 ℃.Result:TLC could be used for the identification of Semen Zizyphi Spinosae
the spots were clear and accurate.The jujuboside A was linear with-in the range of 0.6-3.0 μg(r = 0.999 9).The average recovery was 99.98% and RSD was 1.19%.Conclusion:These methods are sensitive
accurate and simple.The methods can be used for the quality control of Suanzaoren He-huan Formula.
Determination of Astragnaloside IV in Baicaojing Oral Solution by HPLC-ELSD
Study on Quality Standard of Semen Descurainiae Tostum
Study on Quality Standard of Dingxiang-Shidi Tablet
Related Author
LIU Jing-shu
QIAO Wei
HAO Lan-fang
LI Lu
YANG Yi
SUN Jian1
LIU Hong2
FAN Bin1
Related Institution
Shandong University of Traditional Chinese Medicine
Henan Engineering Research Center for Quality Control and Evaluation of Traditional Chinese Medicine(TCM),School of Pharmacy,Henan University of Chinese Medicine
Luohe Medical College
Third Affiliated Hospital of Henan University of Chinese Medicine
Tianjin University of Traditional Chinese Medicine