Determination of D-pinitol in Lespedeza cuneata by HPLC-ELSD

CAI Ling1; HUANG Jian1; LV Yang2; ZHOU Bin2; CHEN Long-sheng2

doi:10.13422/j.cnki.syfjx.2011.05.049

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Determination of D-pinitol in Lespedeza cuneata by HPLC-ELSD

更新时间：2024-09-23

- Determination of D-pinitol in Lespedeza cuneata by HPLC-ELSD
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 5, Pages: 80-82(2011)
- 作者机构：
  
  1. 中南大学化学化工学院
  2. 安徽省科学技术研究院
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2011.05.049
  CLC： R284.1
- Published：2011
- 稿件说明：
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[1]蔡玲,黄坚,吕杨,周斌,陈龙胜.HPLC-ELSD法测定夜关门中D-松醇的含量[J].中国实验方剂学杂志,2011,17(05):80-82.

CAI Ling1, HUANG Jian1, LV Yang2, et al. Determination of D-pinitol in Lespedeza cuneata by HPLC-ELSD[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(5): 80-82.
[1]蔡玲,黄坚,吕杨,周斌,陈龙胜.HPLC-ELSD法测定夜关门中D-松醇的含量[J].中国实验方剂学杂志,2011,17(05):80-82. DOI： 10.13422/j.cnki.syfjx.2011.05.049.

CAI Ling1, HUANG Jian1, LV Yang2, et al. Determination of D-pinitol in Lespedeza cuneata by HPLC-ELSD[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(5): 80-82. DOI： 10.13422/j.cnki.syfjx.2011.05.049.

摘要

目的:建立高效液相色谱-蒸发光散射检测器（HPLC-ELSD）测定夜关门中D-松醇含量的方法。方法:样品用乙醇-水（70∶30）提取1.0 h后过滤

滤渣再用63%的乙醇-水提取1 h过滤

合并2次滤液并浓缩

定容;采用Cosmosil Sugar-D柱进行分离

乙腈-水（70∶30）为流动相

流速为1 mL·min-1

ELSD漂移管温度55℃

载气为氮气。结果:D-松醇在0.377～1.844μg呈现良好的线性关系（r=0.999 5）

精密度为0.22%

加标回收率为96.78%。结论:该方法操作简单、准确度和灵敏度高、适合于D-松醇的检测和含量测定。

Abstract

Objective:An analytical method was developed for determining D-pinitol in Lespedeza cuneata by HPLC-ELSD.Method:Sample was extracted by ethanol-water solution（70:30 by volume） for 1.0 h

then filtered and the residue was extracted by ethanol-water solution（63:37 by volume） for another hour

filtered again and combined the filtrate then concentrated it

and diluted it into 100 mL.The separation was performed on a Cosmosil-sugar D column with the mobile phase consisting of water and acetonitrile（20:80 by volume）.And the flow rate was 1 mL·min-1

the temperature of drift tube was55 ℃ with Nitrogen as developing solvent.The linear calibration curve was obtained in the range of 0.377-1.844 μg with a linear correlation coefficient（r） of 0.999 5.The RSD is 0.22%

and the add mark recovery was 96.78%.This method is simple

high sensitivity and good precision

it is suitable for determation D-pinitol in Lespedeza cuneata.

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