Simultaneous Determination of Five Active Compounds in Geranium sibiricum by HPLC

LUO Hong; YIN Hai-bo

doi:10.13422/j.cnki.syfjx.2011.05.050

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Simultaneous Determination of Five Active Compounds in Geranium sibiricum by HPLC

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- Simultaneous Determination of Five Active Compounds in Geranium sibiricum by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 5, Pages: 83-86(2011)
- 作者机构：
  
  辽宁中医药大学药学院
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2011.05.050
  CLC： R284.1
- Published：2011
- 稿件说明：
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[1]罗宏,尹海波.HPLC同时测定鼠掌老鹳草中5种活性成分的含量[J].中国实验方剂学杂志,2011,17(05):83-86.

LUO Hong, YIN Hai-bo. Simultaneous Determination of Five Active Compounds in Geranium sibiricum by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(5): 83-86.
[1]罗宏,尹海波.HPLC同时测定鼠掌老鹳草中5种活性成分的含量[J].中国实验方剂学杂志,2011,17(05):83-86. DOI： 10.13422/j.cnki.syfjx.2011.05.050.

LUO Hong, YIN Hai-bo. Simultaneous Determination of Five Active Compounds in Geranium sibiricum by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(5): 83-86. DOI： 10.13422/j.cnki.syfjx.2011.05.050.

摘要

目的:建立HPLC同时测定鼠掌老鹳草中没食子酸

原儿茶酸

槲皮素

山奈素

山奈素-3

7-O-α-二鼠李糖苷的含量测定方法。方法:采用RP-HPLC

Agilent TC-C18色谱柱（4.6 mm×250 mm

5μm）

0.4%磷酸水溶液-甲醇为流动相梯度洗脱

流速0.8 mL·min-1

检测波长为切换波长

0～23 min

270 nm;23～55 min

370 nm

柱温30℃。结果:没食子酸

原儿茶酸

槲皮素

山奈素

山奈素-3

7-O-α-二鼠李糖苷的线性范围0.192～1.732 g·L-1（r=0.999 7）

0.016～0.256 g·L-1（r=0.999 9）

0.004～0.039 g·L-1（r=0.999 9）

0.004～0.041 g·L-1（r=0.999 9）

0.200 4～1.603 2 g·L-1（r=0.999 8）

平均回收率（n=3）分别为99.93%（RSD 1.5%）

98.27%（RSD 1.7%）

100.07%（RSD 2.2%）

99.92%（RSD 1.8%）

99.63%（RSD1.0%）。结论:该含量测定的方法快速、准确、可靠、重复性好

可为药材的质量控制提供依据。

Abstract

Objective:To determine 5 active compounds in Geranium sibiricum

namely gallic acid

protocat-echuin acid

quercetin

kaempferol-7-O-rhamnoside and kaemferitrin.Method:The HPLC method was applied and the separation was performed on an Agilent TC-C18 analytical column（4.6 mm × 250 mm

5 μm）.The mobile phase was methanol（A）-water containing 0.4% H3PO4（B） with gradient elution mode at the flow rate of 0.9 mL·min-1.The detection was set at 270 nm（0-23 min）

370 nm（23-55 min）

and the column temperature was 30 ℃.Result:The liner ranges of gallic acid

protocatechuin acid

quercetin

kaempferol-7-O-rhamnoside and kaemferitrin were 0.192-1.732 g·L-1（r = 0.999 7）

0.016-0.256 g·L-1（r = 0.999 9）

0.004-0.039 g·L-1（r = 0.999 9）

0.004-0.041 g·L-1（r = 0.999 9）

0.200 4-1.603 2 g·L-1（r = 0.999 8）.The average recoveries（n = 3） were 99.93%（RSD 1.5%）

97.27%（RSD 1.7%）

100.07%（RSD 2.2%）

99.92%（RSD 1.8%）

99.63%（RSD 1.0%）.Conclusion:The methord is simple and accurate with a good reproducibility and can be used as a quality control methord for G.sibiricum of different sources.

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