CHEN Tian-chao1, ZHAI Lai-chao2. HPLC Simultaneously Determination of Content About Catalpol and Verbascoside in Rehmannia[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(5): 105-107.
CHEN Tian-chao1, ZHAI Lai-chao2. HPLC Simultaneously Determination of Content About Catalpol and Verbascoside in Rehmannia[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(5): 105-107. DOI: 10.13422/j.cnki.syfjx.2011.05.056.
HPLC Simultaneously Determination of Content About Catalpol and Verbascoside in Rehmannia
摘要
目的:研究地黄中梓醇与毛蕊花糖苷的含量测定方法
为其质量评价提供依据。方法:采用HPLC梯度洗脱法
在同一色谱条件下测定含量。色谱条件:AgilentSB-AQ色谱柱(4.6 mm×250 mm
5μm)
流速1 mL·min-1
柱温25℃
检侧波长210 nm。结果:两种成分均能达到基线分离
线性良好。结论:经过系统的方法学考察
该方法操作简便、结果准确、重现性好
适用于地黄2010年版药典规定的这2种苷类成分的含量测定。
Abstract
Objective:To study the determination method about the catalpol and verbascoside
provide basis for quality evaluation.Method:Gradient elution was applied in HPLC analysis.Agilent SB-AQ chromatographic column(4.6 mm × 250 mm
5 μm)
velocity 1mL·min-1
column temperature was 25 ℃
wavelength was 210 nm.Result:Two components can achieve good baseline separation
linear.Conclusion:After the systematic methodology
the method is simple
accurate
reproducible
applicable to the provisions by pharmacopoeia rehmannia glutinosa two content determination.
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Related Author
CHEN Tian-chao
ZHAI Lai-chao
Mei-hua WANG
Han-tao ZHAO
Meng-xue PAN
Lyu-jiang YUAN
Jie-yao HUANG
Dan-ning LIU
Related Institution
College of Pharmaceutical Sciences, Southwest University
Henan Engineering Research Center for Quality Control and Evaluation of Traditional Chinese Medicine(TCM),School of Pharmacy,Henan University of Chinese Medicine
Luohe Medical College
Third Affiliated Hospital of Henan University of Chinese Medicine
Tianjin University of Traditional Chinese Medicine