TAN Li-ping1, TAN Xiao-yong2. Determination of Adenosine in Pegasus Laternarius Cuvier by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(5): 107-109.
TAN Li-ping1, TAN Xiao-yong2. Determination of Adenosine in Pegasus Laternarius Cuvier by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(5): 107-109. DOI: 10.13422/j.cnki.syfjx.2011.05.057.
Determination of Adenosine in Pegasus Laternarius Cuvier by HPLC
摘要
目的:建立海麻雀中腺苷的含量测定方法。方法:采用高效液相色谱法
流动相为磷酸盐缓冲液(pH 6.5)-甲醇(80∶20)
色谱柱为依利特HypersiL ODS-C18(4.6 mm×250 mm
5μm)
流速1.0 mL·min-1
检测波长260 nm。结果:腺苷在0.128 3~0.342 1μg呈良好线性关系
平均加样回收率98.4%
RSD 1.1%。结论:方法操作简单
结果可靠
重复性好
可以为海麻雀质量控制提供科学依据。
Abstract
Objective:To establish a method for content determination of adenosine in Pegasus laternarius Cuvier.Method:HPLC conditions were as follow:the mobile phase was phosphate buffer saline(pH 6.5)-methanol(80:20)
Hypersil ODS-C18 column(4.6 mm × 250 mm
5 μm)
volume flow 1.0 mL·min-1
wave length at 260 nm.Result:Linearity was found in the range of 0.128 3-0.342 1 μg
the average recovery was 98.4% and RSD was 1.1%.Conclusion:The results show that this method is simple
accurate and reproducible
it call be used for quality control in P.laternarius.
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Related Author
TAN Li-ping
TAN Xiao-yong
MAO Yan
YANG Wei-jun
MA Yila·maimaitiyiming
HE Jin-hua
ZHOU Cheng-hui
LI Chun-xue
Related Institution
Xinjiang Institute of Materia Medica,Urmiqu
Key Laboratory of Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences
Jiangxi Guoyao Co. Ltd.
Jilin Agricultural University
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences