HUANG Jiang-jian1, GAO Ying2, LI Wei-ming1, et al. Quantitative Determination of Dioscin in Bulbus Lilii by HPLC-ELSD[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(5): 110-112.
HUANG Jiang-jian1, GAO Ying2, LI Wei-ming1, et al. Quantitative Determination of Dioscin in Bulbus Lilii by HPLC-ELSD[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(5): 110-112. DOI: 10.13422/j.cnki.syfjx.2011.05.058.
Quantitative Determination of Dioscin in Bulbus Lilii by HPLC-ELSD
摘要
目的:采用蒸发光散射检测器(ELSD)测定百合中薯蓣皂苷的含量。方法:以AKZONOBEL Kromasil C18(4.6 mm×250 mm
5μm)为色谱柱
乙腈-0.2%甲酸(45∶55)为流动相
流速1.0 mL·min-1
漂移管的温度46℃
载气(N2)1.56 bar。结果:薯蓣皂苷在3.31~26.5μg有良好线性关系
平均回收率为99.2%
RSD 1.02%。结论:该方法简单
分离效果好
结果准确
可用于百合药材中薯蓣皂苷的含量测定。
Abstract
Objective:To estahlish a method for the determination of the content of dioscin in Bulbus Liliiusing high-performance liquid chromatography with evaporative light scattering detector(HPLC-ELSD).Method:HPLC-ELSD analysis was performed on Akzonobel Kromasil C18(4.6 mm × 250 mm
5 μm)with a mixture of acetonitrile(A) and 0.2% formic acid water(B)(45:55) as the mobile phase.Drift tube temperature was 46 ℃
pressure of nebulizer gas was 1.56 bar.Result:The resolution of dioscin was good.The linear calibration curves were obtained over the range of 3.31-26.5 μg.The average recovery of the method was 99.23% with RSD of 1.02%.Conclusion:This method is convenient
reliable and can be used reliably for analyzing dioscin in bulbus Lilii.