WU Jun-zhu, ZHOU Nong, ZHOU Mei, et al. Determination of Shikimic Acid in Songling Xuemaikang Capsules by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(5): 113-115.
WU Jun-zhu, ZHOU Nong, ZHOU Mei, et al. Determination of Shikimic Acid in Songling Xuemaikang Capsules by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(5): 113-115. DOI: 10.13422/j.cnki.syfjx.2011.05.059.
Determination of Shikimic Acid in Songling Xuemaikang Capsules by RP-HPLC
摘要
目的:采用RP-HPLC测定松龄血脉康胶囊中莽草酸的含量。方法:色谱柱为Venusil XBP-C18色谱柱(4.6 mm×250 mm
5μm)
流动相甲醇-1%磷酸水溶液(3∶97)
检测波长214 nm
流速0.8 mL·min-1
柱温25℃。结果:莽草酸在0.4~8.0μg呈良好的线性关系
r=0.999 8
平均回收率为98.87%。结论:该方法准确、可靠、重复性好
可为松龄血脉康胶囊的质量控制提供参考。
Abstract
Objective:To establish a RP-HPLC method for determination of the content of shikimic acid in Songling Xuemaikang capsules.Method:The separation was performed on a Venusil XBP-C18 column(4.6 mm ×250 mm
5 μm) and detected at 214 nm at the flow rate of 0.8 mL·min-1 using methanol-1% phosphoric acid (3:97) as the mobile phase.Column temperature was at 25 ℃.Result:The good linear relation was showed in the ranges of 0.4-8.0 μg(r = 0.999 8) for shikimic acid.The recovery was 98.87%.Conclusion:The method had been successfully applied to determine shikimic acid in Songling Xuemaikang capsules.It can be used for quality control of Songling Xuemaikang capsules.