Determination of Caffeic Acid,Ferulic Acid,Rosmarinic Acid in Rosmarinus officinalis by HPLC with Changing Ultraviolet-visible Wavelength

WANG Hui; ZHANG Zhen-qiu

doi:10.13422/j.cnki.syfjx.2011.05.060

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Determination of Caffeic Acid,Ferulic Acid,Rosmarinic Acid in Rosmarinus officinalis by HPLC with Changing Ultraviolet-visible Wavelength

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- Determination of Caffeic Acid,Ferulic Acid,Rosmarinic Acid in Rosmarinus officinalis by HPLC with Changing Ultraviolet-visible Wavelength
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 5, Pages: 116-118(2011)
- 作者机构：
  
  辽宁中医药大学
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2011.05.060
  CLC： R284.1
- Published：2011
- 稿件说明：
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[1]王珲,张振秋.HPLC波长切换法同时测定迷迭香中咖啡酸、阿魏酸和迷迭香酸的含量[J].中国实验方剂学杂志,2011,17(05):116-118.

WANG Hui, ZHANG Zhen-qiu. Determination of Caffeic Acid,Ferulic Acid,Rosmarinic Acid in Rosmarinus officinalis by HPLC with Changing Ultraviolet-visible Wavelength[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(5): 116-118.
[1]王珲,张振秋.HPLC波长切换法同时测定迷迭香中咖啡酸、阿魏酸和迷迭香酸的含量[J].中国实验方剂学杂志,2011,17(05):116-118. DOI： 10.13422/j.cnki.syfjx.2011.05.060.

WANG Hui, ZHANG Zhen-qiu. Determination of Caffeic Acid,Ferulic Acid,Rosmarinic Acid in Rosmarinus officinalis by HPLC with Changing Ultraviolet-visible Wavelength[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(5): 116-118. DOI： 10.13422/j.cnki.syfjx.2011.05.060.

摘要

目的:建立迷迭香中咖啡酸、阿魏酸和迷迭香酸的含量测定方法

为其质量标准的研究提供科学依据。方法:采用高效液相色谱切换波长法同时测定咖啡酸、阿魏酸、迷迭香酸的含量。色谱条件为phenomsil C18（4.6 mm×250 mm

5μm）分析柱进行测定

流动相甲醇-0.1%磷酸水溶液（32∶68）;流速1.0 mL·min-1

检测波长0～20 min为323 nm

20～30 min为316 nm

30 min为329 nm。结果:此方法线性良好

咖啡酸

阿魏酸和迷迭香酸的平均加样回收率分别为103.7%

99.5%

101.7%;RSD分别为1.5%

1.2%

1.5%。结论:本方法简便

准确

重复性好

可做为迷迭香质量控制的定性依据。

Abstract

Objective:To establish the method for determining the content of caffeic acid

ferulic acid

ros-marinic acid in Rosmarinus officinalis for its quality standards to provide the scientific basis for the study.Caffeic acid

ferulic acid

rosmarinic acid were determined by HPLC simultaneously with changing ultraviolet-visible wave-length.Chromatographic condition was composed of C18（4.6 mm × 250 mm

5 μm）

mobile phase methanol – 0.1% phosphoric acid（32:68）;Flow rate was 1.0 mL·min-1;the detection wavelength of caffeic acid at 323 nm

Ferulic acid at 316 nm and rosmarinic acid at 329 nm.the isolation effect among caffeic acid

ferulic acid

and ros-marinic acid showed good linear correlation

the average recoveries were 103.7%

99.5%

101.7%;RSD were 1.5%

1.2%

1.5%.the method and was convenient

accurate and good reappearance

It can be used as quantity basis of the quality control of R.officinalis.

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Related Institution

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