MEI Xue, LI Jian-sheng, ZHANG Yan-xia. Influence of Dusuqing on Expression of Inflammatory Factor in Lung Tissue of Rat Model with Bacterial Pneumonia and Phlegm-heat Syndrome[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(5): 153-156.
MEI Xue, LI Jian-sheng, ZHANG Yan-xia. Influence of Dusuqing on Expression of Inflammatory Factor in Lung Tissue of Rat Model with Bacterial Pneumonia and Phlegm-heat Syndrome[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(5): 153-156. DOI: 10.13422/j.cnki.syfjx.2011.05.069.
Influence of Dusuqing on Expression of Inflammatory Factor in Lung Tissue of Rat Model with Bacterial Pneumonia and Phlegm-heat Syndrome
Objective:To investigate influence of Dusuqing(DSQ)
a traditional Chinese medicine prescip-tion
on expression of inflammatory factor in lung tissue of rat models with bacterial pneumonia and phlegm-heat syn-drome and to reveal the mechanism of DSQ in treatment of the disease.Method:Wistar rats were randomized into five groups:normal
pneumonia
pneumonia with phlegm-heat syndrome
positive control and DSQ treated groups.Rat models with bacterial pneumonia accompanied by Phlegm-heat syndrome were made.The expressions of IL-1β
TNF-α
IL-6 and IL-10 proteins in lung tissue were analyzed by immunohistochemistry staining.The expression of IL-1βmRNA
IL-10mRNA was measured by RT-PCR.Result:The expressions of IL-1β
TNF-α
IL-6
IL-10
IL-1βmRNA and IL-10mRNA in pneumonia group and pneumonia with phlegm-heat syndrome group were markedly higher than those in normal control group(P < 0.01).And in pneumonia with phlegm-heat syndrome group the ex-pressions of TNF-α
IL-6
IL-10 proteins and IL-10 mRNA were significantly higher than those in the pneumonia group(P < 0.01
P < 0.05).Compared with pneumonia with phlegm-heat syndrome group
the expressions of IL-1β
TNF-α
IL-6 and IL-1β mRNA were markedly weakened in positive control group and DSQ treated group(P < 0.01) and the expressions in DSQ treated group were weaked significantly than those in positive control group(P < 0.05).Compared with pneumonia with phlegm-heat syndrome group
the expressions of IL-10 protein and mRNA were en-hanced in DSQ treated group(P < 0.05).Conclusion:The unbalance between proinflammatory and anti-inflammato-ry factor is involved in the pathological damage of lung tissues in pneumonia with phlegm-heat syndrome.The mecha-nism of DSQ in treating bacterial pneumonia with phlegm-heat syndrome may be related to its ability of inhibiting ex-cessive release of proinflammatory cytokine and promoting IL-10 secretion to regulate the balance between pro-and anti-inflammatory responses.
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Related Author
MEI Xue
LI Jian-sheng
ZHANG Yan-xia
WANG Lu
WANG Jiajia
LIU Jiamin
LI Jiansheng
GAO Xiu-lan
Related Institution
Collaborative Innovation Center for Chinese Medicine and Respiratory Diseases Co-constructed by Henan Province and Education Ministry of P.R. China/Henan Key Laboratory of Chinese Medicine for Respiratory Disease,Henan University of Chinese Medicine
The First Affiliated Hospital of Henan University of Chinese Medicine
The First Clinical Medical School of Henan University of Chinese Medicine