LIU Ming1, SUN Jian-ning1, DONG Shi-fen1, et al. Changes in Brain Energy Metabolism for Different Duration of Cerebral Ischemia in Rats[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(5): 216-218.
LIU Ming1, SUN Jian-ning1, DONG Shi-fen1, et al. Changes in Brain Energy Metabolism for Different Duration of Cerebral Ischemia in Rats[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(5): 216-218. DOI: 10.13422/j.cnki.syfjx.2011.05.082.
Changes in Brain Energy Metabolism for Different Duration of Cerebral Ischemia in Rats
摘要
目的:研究大鼠脑缺血不同时间脑能量代谢的变化。方法:复制大鼠永久性局灶性大脑中动脉缺血模型
测定缺血6
24
72
168
336 h脑组织乳酸(Lac)和丙酮酸(Pyru)的含量
Na+
K+-ATPase和Ca2+
Mg2+-ATPase活性。结果:在各个不同时间点
各检测指标与假手术组比较均具有显著性
P<0.05
P<0.01。结论:在缺血336 h内观测脑组织中能量代谢相关指标
可以用于评价药物对脑缺血的保护作用。
Abstract
Objective:To investigate changes in brain energy metabolism for different durations of cerebral ischemia in rats.Method:Permanent cerebral ischemia was induced by permanent middle cerebral artery occlusion(pMCAO) in rats
and ischemic cortex Lac and Pyru content
Na +
K +-ATPase and Ca2 +
Mg2 +-ATPase activity were measured at 6
24
72
168
336 h after ischemia.Result:At different time points after ischemia
brain energy metabolism in vehicle-treated rats showed significant changes compared with sham-operated group(P < 0.05
P < 0.01).Conclusion:There were fluctuation changes in ischemic cortex Lac and Pyru content
Na +
K +-ATPase and Ca2 +
Mg2 +-ATPase activity at different time points after ischemia in rats in vehicle-treated groups.Changes of is-chemic cortex Lac and Pyru content
Na +
K +-ATPase and Ca2 +
Mg2 +-ATPase activity may be used as important indexes for early diagnosis and treatment assessment of cerebral ischemia.