Extraction and Purification of Total Iridoid Glycosidesfrom Herba Lamiophlomis Rotata in Industrialization

QIU Jian-guo; ZHANG Quan-long; WEI Li-li; LI Mao-xing; JIA Zheng-ping; ZHANG Ru-xue; FAN Peng-cheng; QIU Yi-nong

doi:10.13422/j.cnki.syfjx.2011.13.001

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Extraction and Purification of Total Iridoid Glycosidesfrom Herba Lamiophlomis Rotata in Industrialization

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- Extraction and Purification of Total Iridoid Glycosidesfrom Herba Lamiophlomis Rotata in Industrialization
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 13, Pages: 32-35(2011)
- 作者机构：
  
  兰州军区兰州总医院药材科
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2011.13.001
  CLC： TQ461
- Published：2011
- 稿件说明：
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[1]邱建国,张泉龙,尉丽力,李茂星,贾正平,张汝学,樊鹏程,邱宜农.工业化提取分离纯化独一味总环烯醚萜苷类成分[J].中国实验方剂学杂志,2011,17(13):32-35.

QIU Jian-guo, ZHANG Quan-long, WEI Li-li, et al. Extraction and Purification of Total Iridoid Glycosidesfrom Herba Lamiophlomis Rotata in Industrialization[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(13): 32-35.
[1]邱建国,张泉龙,尉丽力,李茂星,贾正平,张汝学,樊鹏程,邱宜农.工业化提取分离纯化独一味总环烯醚萜苷类成分[J].中国实验方剂学杂志,2011,17(13):32-35. DOI： 10.13422/j.cnki.syfjx.2011.13.001.

QIU Jian-guo, ZHANG Quan-long, WEI Li-li, et al. Extraction and Purification of Total Iridoid Glycosidesfrom Herba Lamiophlomis Rotata in Industrialization[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(13): 32-35. DOI： 10.13422/j.cnki.syfjx.2011.13.001.

摘要

目的:应用大孔吸附树脂结合聚酰胺柱色谱法富集分离独一味中总黄酮、总环烯醚萜苷类极性成分

实现工业化生产。方法:用系列色谱设备进行分离富集

一阶导数紫外分光光度法测定总环烯醚萜苷含量、高效液相色谱法测定山栀苷甲酯

8-O-乙酰山栀苷甲酯

并考察不同洗脱条件下有效成分总环烯醚萜苷的变化。结果:采用70%乙醇溶液为溶媒。大孔吸附树脂柱用"下进上出"的进样方式和"上进下出"的洗脱方式时

得率为20.80%

总环烯醚萜苷、山栀苷甲酯和8-O-乙酰山栀苷甲酯的转移率分别为70.77%

76.36%

68.74%。结论:生产工艺方法及参数可以成功地将实验成果转化为工业化生产。

Abstract

Objective:The macroporous adsorptive resins chromatotraphic column combining with polyamide chromatographic column was used to separate the flavonoides

iridoid glycosides and maximum polarity ingredients in industrialization.Method: The flavonoides

iridoid glycosides and maximum polarity ingredients were separated and enriched by CCPP serial laminar analysis equipment

the content of total iridoid glycosides was assayed by the first derivative spectrophotometry;the content of shanzhiside methylester and 8-O-acetyl shanzhiside methylester was determined by HPLC and the change of total iridoid glycosides was investigated in different elution requirement.Result: 70% alcohol was used as dissolvent.Using the ’introduction from inferior surface’ sample introduction mode and ’introduction from superior surface’ eluting mode in the macroporous adsorptive resins

the yield was 20.80%

transfusion ratio for shanzhiside methylester and 8-O-acetyl shanzhiside methylester was 70.77% 73.36% and 68.74% respectively.Conclusion: This production technology may transform experimental achievement to industrial production successfully.

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