HPLC Fingerprint Analysis on Yinqiao Chaigui Decoction

HE Jian-xiong1; LAI Xiao-ping1; WEI Gang1; ZUO Jun-ling2

doi:10.13422/j.cnki.syfjx.2011.13.037

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HPLC Fingerprint Analysis on Yinqiao Chaigui Decoction

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- HPLC Fingerprint Analysis on Yinqiao Chaigui Decoction
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 13, Pages: 63-67(2011)
- 作者机构：
  
  1. 广州中医药大学
  2. 广州中医药大学第一附属医院
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2011.13.037
  CLC： R284.1
- Published：2011
- 稿件说明：
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[1]何建雄,赖小平,魏刚,左竣岭.银翘柴桂汤HPLC指纹图谱研究[J].中国实验方剂学杂志,2011,17(13):63-67.

HE Jian-xiong1, LAI Xiao-ping1, WEI Gang1, et al. HPLC Fingerprint Analysis on Yinqiao Chaigui Decoction[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(13): 63-67.
[1]何建雄,赖小平,魏刚,左竣岭.银翘柴桂汤HPLC指纹图谱研究[J].中国实验方剂学杂志,2011,17(13):63-67. DOI： 10.13422/j.cnki.syfjx.2011.13.037.

HE Jian-xiong1, LAI Xiao-ping1, WEI Gang1, et al. HPLC Fingerprint Analysis on Yinqiao Chaigui Decoction[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(13): 63-67. DOI： 10.13422/j.cnki.syfjx.2011.13.037.

摘要

目的:建立银翘柴桂汤HPLC指纹图谱分析方法

拟定其指纹特征图谱指标成分群。方法:采用HPLC测定

依利特ODS柱（4.6 mm×50 mm

5μm）

流动相甲醇-0.1%磷酸溶液（梯度洗脱）

检测波长230 nm

流速1 mL·min-1

柱温35℃。结果:10批银翘柴桂汤中共标示出17个共有峰

全方汤剂的特征峰基本为各味药特征峰的加和;利用指纹图谱相似度评价系统软件

测得10批汤剂指纹图谱相似度在0.910～0.986。结论:方法准确可靠

重复性好

可为银翘柴桂颗粒剂的开发研究提供质量评价依据

并为其物质基础研究提供了一定参考。

Abstract

Objective:To establish the method of fingerprint analysis on Yinqiao Chaigui Decoction

work out the characteristic fingerprint

and study the influence of various compatibilities on fingerprint peaks.Method:HPLC with Hypersil ODS was used

methanol-water（gradient elution）as a mobile phase and detection wavelength at 230 nm

flow rate was 1 mL·min-1

and column temperature was 35 ℃.Result:Seventeen common peaks were separated in 10 batches of Yinqiao Chaigui Decoction.A little influence on characteristic peaks was found in various compatibilities

but there was no new characteristic peak.The characteristic peaks were the summation

peak 2（chlorogenic acid）

7 were from Lonicera japonica

peak14（baicalin）

17 were from Scutellaria baicalersis;peak 5（paeoniflorin） was from Paeonia lactiflora Pall;peak 11

12 were from Cinnamomum cassia presl and peak 1

11 were from Forsythia suspense.Conclusion:The method is reliable

accurate and provides a reference for the rules of the compatibility and material base of Yinqiao Chaigui Decoction.

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Related Author

HE Jian-xiong

LAI Xiao-ping

WEI Gang

ZUO Jun-ling

Mei WU

Hong-zhe LI

Yong-mei XUE

Qing-zhi ZHANG

Related Institution

College of Pharmaceutical Science, Yunnan University of Traditional Chinese Medicine

Henan University of Chinese Medicine

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College of Pharmacy,Dali University,Dali

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