Comparison of Content of Secoiridoids in Wild Gentiana rigescens from Different Places

SHEN Tao 1; JIN Hang1; YANG Tao1; YANG Mei-quan1; YANG Wei-ze2; WANG Yuan-zhong2; ZHANG Jin-yu1

doi:10.13422/j.cnki.syfjx.2011.13.039

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Comparison of Content of Secoiridoids in Wild Gentiana rigescens from Different Places

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- Comparison of Content of Secoiridoids in Wild Gentiana rigescens from Different Places
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 13, Pages: 70-73(2011)
- 作者机构：
  
  1. 云南省农业科学院药用植物研究所
  2. 云南大学生命科学学院
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2011.13.039
  CLC： R284.1
- Published：2011
- 稿件说明：
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[1]沈涛,金航,杨涛,杨美权,杨维泽,王元忠,张金渝.不同产地野生滇龙胆中主要裂环烯醚萜类成分的含量比较[J].中国实验方剂学杂志,2011,17(13):70-73.

SHEN Tao 1, JIN Hang1, YANG Tao1, et al. Comparison of Content of Secoiridoids in Wild Gentiana rigescens from Different Places[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(13): 70-73.
[1]沈涛,金航,杨涛,杨美权,杨维泽,王元忠,张金渝.不同产地野生滇龙胆中主要裂环烯醚萜类成分的含量比较[J].中国实验方剂学杂志,2011,17(13):70-73. DOI： 10.13422/j.cnki.syfjx.2011.13.039.

SHEN Tao 1, JIN Hang1, YANG Tao1, et al. Comparison of Content of Secoiridoids in Wild Gentiana rigescens from Different Places[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(13): 70-73. DOI： 10.13422/j.cnki.syfjx.2011.13.039.

摘要

目的:采用高效液相色谱法测定不同产地野生滇龙胆中龙胆苦苷、獐牙菜苦苷及獐牙菜苷的含量。方法:岛津Shim-pack VP-ODS柱（4.6 mm×150 mm

5μm）

流动相甲醇-水（30∶70）

流速1.0 mL·min-1

龙胆苦苷检测波长270 nm

獐牙菜苦苷及獐牙菜苷检测波长245 nm

柱温25℃。结果:龙胆苦苷、獐牙菜苦苷、獐牙菜苷的线性范围为0.4～2 mg·L-1;相关系数分别为0.999 7

0.999 6

0.998 7;回收率分别为100.04%（RSD 1.49%）

98.48%（RSD 1.67%）

98.46%（RSD 1.15%）结论:用本法测定不同产地野生滇龙胆中3种成分含量

方法可行

结果可靠

为野生滇龙胆资源的开发与利用提供资料。

Abstract

Objective:To develop an HPLC method for determining the content of gentiopicroside

swertiamarin and sweroside in wild Gentiana rigescens.Method: Column was Shim-pack VP-ODS（4.6 mm×150 mm

5 μm）.The mobile phase consisted of CH3OH and water（30∶70）.Flow rate was 1.0 mL·min-1 at 25 ℃.Detecting wavelength of gentiopicroside was at 270 nm while swertiamarin and sweroside were detected at 245 nm.Result: The linear rang of calibration curve was 0.4-2 g·L-1 with the relation coefficient of 0.999 7

0.999 6 and 0.998 7 respectively.The average recovery of three active constituents was 100.04%（RSD 1.49%）

98.48%（RSD 1.67%）and 98.46%（RSD 1.15%）.Conclusion: The method is accurate and can supply basis for the exploitation and utilization of Gentiana rigescens resources.

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Related Author

SHEN Tao

JIN Hang

YANG Tao

YANG Mei-quan

YANG Wei-ze

WANG Yuan-zhong

ZHANG Jin-yu

WANG Shuang

Related Institution

Guilin Medical University

School of Pharmaceutical Sciences, Zhengzhou University

The First Affiliated Hospital of Zhengzhou University

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Jilin Agricultural University

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