Determination of Hypoxanthine and Uridine in Leech by HPLC

WANG Yan1; YANG Pei-min2; DAI Long1; LIU Guo-fei3

doi:10.13422/j.cnki.syfjx.2011.13.040

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Determination of Hypoxanthine and Uridine in Leech by HPLC

更新时间：2024-09-23

- Determination of Hypoxanthine and Uridine in Leech by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 13, Pages: 74-76(2011)
- 作者机构：
  
  1. 山东中医药大学
  2. 山东中医药大学附属医院
  3. 北京和润创新医药科技发展有限公司
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2011.13.040
  CLC： R284.1
- Published：2011
- 稿件说明：
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[1]王艳,杨培民,代龙,刘国飞.HPLC测定水蛭仿生酶解有效部位中次黄嘌呤及尿苷[J].中国实验方剂学杂志,2011,17(13):74-76.

WANG Yan1, YANG Pei-min2, DAI Long1, et al. Determination of Hypoxanthine and Uridine in Leech by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(13): 74-76.
[1]王艳,杨培民,代龙,刘国飞.HPLC测定水蛭仿生酶解有效部位中次黄嘌呤及尿苷[J].中国实验方剂学杂志,2011,17(13):74-76. DOI： 10.13422/j.cnki.syfjx.2011.13.040.

WANG Yan1, YANG Pei-min2, DAI Long1, et al. Determination of Hypoxanthine and Uridine in Leech by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(13): 74-76. DOI： 10.13422/j.cnki.syfjx.2011.13.040.

摘要

目的:测定水蛭仿生酶解有效部位中次黄嘌呤及尿苷的含量

为其质量控制提供科学依据。方法:采用SinoChrom ODS-BP液相色谱柱

以甲醇-水为流动相

测定波长254 nm。结果:次黄嘌呤在0.88～14.08 g·L-1呈良好线性关系（r=0.9997）

平均加样回收率为99.69%

RSD 1.23%;尿苷在2.12～33.92 g·L-1呈良好线性关系（r=0.999 8）

平均加样回收率为99.20%

RSD 1.17%。结论:该法操作简便

结果可靠

为水蛭仿生酶解有效部位的质量研究提供有效的方法。

Abstract

Objective:To determine hypoxanthine and uridine in leech to provide scientific basis for its quality control.Method: An ODS-BP liquid chromatographic column was used.The mobile phase was methanol-water.Wavelength was at 254 nm.Result: The linear range of hypoxanthine was within 0.88-14.08g·L-1（r=0.999 7）.The sample recovery rate was 99.69%

RSD 1.23%.The linear range of uridine was within 2.12-33.92 g·L-1（r=0.999 8）.The sample recovery rate was 99.20%

RSD 1.17%.Conclusion: This method has good repeatability and flexibility.It can be used for quality control in production of leech.

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Related Author

WANG Yan

YANG Pei-min

DAI Long

LIU Guo-fei

XU Gui-hua

ZHANG Ji-huan

QIAO Jian-wei

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Related Institution

Lunan Pharmaceutical Group Co.,Ltd.

State Key Laboratoryof Generic Pharmaceutical Technology for Chinese Traditional Medicine

Key Laboratory of Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences

Jiangxi Guoyao Co. Ltd.

College of Pharmaceutical Science, Yunnan University of Traditional Chinese Medicine

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