SHA La Mai-ti·Ai-li, KAI Sai-er·A Bu-la. Studies on Qualitative and Quantitative Methods for Fufang Kali Ziran Tincture[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(13): 77-80.
SHA La Mai-ti·Ai-li, KAI Sai-er·A Bu-la. Studies on Qualitative and Quantitative Methods for Fufang Kali Ziran Tincture[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(13): 77-80. DOI: 10.13422/j.cnki.syfjx.2011.13.041.
Studies on Qualitative and Quantitative Methods for Fufang Kali Ziran Tincture
Objective:To study the qualitative and quantitative methods for Fufangkaliziran Tincture.Method: Psoralea corylifolia
Vernonia anthelmintica
Angelica sinensis
Cnidium monnieri and Eugenia caryophyllata in Fufang Kali Ziran Tincture were identified with the method of TLC.Psoralen was determined with the method of HPLC.The analytical colomn was Kromasil C18(4.6 mm×250 mm
5 μm)
the mobile phase was methanol-water(50∶50).The flow rate was 1.0 mL·min-1
the UV detector wavelength was at 246 nm
injection volume was 10 μL.Result: The spots of five herbal drugs were well separated and without interference in TLC.Quantitative analysis of HPLC showed that the linear range of psoralen was 0.050 04-0.350 28 μg.The average recovery of psoralen was 98.4%.The RSD(n=9)was 0.9%.Conclusion: The method set up could effectively control the quality of Fufang Kali Ziran Tincture.