FENG Qian-kun, JIA Tian-bao, WANG Pei, et al. Content Determination of Naringin in Zhuanggu Controlled Release Mini-pill by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(13): 92-94.
FENG Qian-kun, JIA Tian-bao, WANG Pei, et al. Content Determination of Naringin in Zhuanggu Controlled Release Mini-pill by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(13): 92-94. DOI: 10.13422/j.cnki.syfjx.2011.13.045.
Content Determination of Naringin in Zhuanggu Controlled Release Mini-pill by HPLC
Objective:To establish a method for determining naringin in Zhuanggu controlled release mini-pill.Method: HPLC was used for determination of naringin in Zhuanggu controlled release mini-pill.The separation was performed on a Agilent SB-C18 column(4.6 mm×250 mm
5 μm)at 27 ℃ with methanol-phosphoric acid(33∶67)as mobile phase.The flow rate was 1.0 mL·min-1
detected at wavelength of 283 nm.Result: Naringin was linear in the range of 0.128 8-0.644 0 μg
r=0.999 9.The average recovery was 100.02% with RSD 0.192%.Conclusion: The method is simple and reproducible for the determination of naringin in decoction of Zhuanggu controlled release mini-pill by HPLC.