LI Yong-hong. Determination of Kaempferol in Bairui Particles by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(13): 97-99.
LI Yong-hong. Determination of Kaempferol in Bairui Particles by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(13): 97-99. DOI: 10.13422/j.cnki.syfjx.2011.13.047.
Determination of Kaempferol in Bairui Particles by HPLC
Objective:To establish a method for determination of kaempferol in Bairui particles.Method: HPLC method was used.The samples were separated by Kromasil ODS column(4.6 mm×250 mm
5 μm)
the mobile phase was methanol-0.4% phosphate(60∶40).The detection wavelength was at 268 nm with a flow rate at 1.0 mL·min-1.Result: The linear range of kaempferol was from 13.10-52.40 g·L-1(r=0.999 7).The average recovery was 99.63% with RSD of 0.66%.Conclusion: The method is simple
accurate
specific
and can be used for quality control of kaempferol in Bairui particles.