Content Determination of Chlorogenic Acid in Mori Cortex by HPLC

XIE Zhen1; ZHONG Ming-Yu 2; ZENG Hai-Sheng1

doi:10.13422/j.cnki.syfjx.2011.13.048

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Content Determination of Chlorogenic Acid in Mori Cortex by HPLC

更新时间：2024-09-23

- Content Determination of Chlorogenic Acid in Mori Cortex by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 13, Pages: 100-103(2011)
- 作者机构：
  
  1. 广西中医学院药学院
  2. 广西中医学院第一附属医院
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2011.13.048
  CLC： R284.1
- Published：2011
- 稿件说明：
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[1]谢臻,钟明玉,曾海生.HPLC测定桑白皮中绿原酸的含量[J].中国实验方剂学杂志,2011,17(13):100-103.

XIE Zhen1, ZHONG Ming-Yu 2, ZENG Hai-Sheng1. Content Determination of Chlorogenic Acid in Mori Cortex by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(13): 100-103.
[1]谢臻,钟明玉,曾海生.HPLC测定桑白皮中绿原酸的含量[J].中国实验方剂学杂志,2011,17(13):100-103. DOI： 10.13422/j.cnki.syfjx.2011.13.048.

XIE Zhen1, ZHONG Ming-Yu 2, ZENG Hai-Sheng1. Content Determination of Chlorogenic Acid in Mori Cortex by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(13): 100-103. DOI： 10.13422/j.cnki.syfjx.2011.13.048.

摘要

目的:建立桑白皮饮片中绿原酸的含量测定方法。方法:利用星点设计法优化供试品溶液的提取条件

采用HPLC测定绿原酸的含量

Diamonsil C18柱（4.6 mm×250 mm

5μm）

流动相乙腈-0.4%磷酸（11∶89）;检测波长327 nm;柱温30℃

流速1 mL·min-1。结果:供试品溶液较佳的提取条件为60%甲醇、提取时间15 min

溶媒比为10倍;绿原酸进样量范围在0.045～0.45μg

峰面积与进样量呈良好的线性关系

回归方程为Y=2 329.028 9X+8.184 1（r=0.999 9）

平均回收率为97.89%

RSD 3.14%。结论:建立的方法简便、重复性好、准确度高

可用于桑白皮饮片的质量控制。

Abstract

Objective:To establish a HPLC method for the content determination of chlorogenic acid in Mori Cortex.Method: The central composite rotatable design was used to optimize extracted conditions.The content of chlorogenic acid in Mori Cortex was determined by HPLC on a Diamonsil C18 column（4.6 mm×250 mm

5 μm）detected at the wavelength of 327 nm with acetonitrile-0.4% phosphoric acid（11∶89）solution as the mobile phase

the flow rate was 1 mL·min-1.Result: The optimum extractable conditions to obtain desirable extraction yields for chlorogenic acid were found at the methanol concentration of 60%

extraction time of 45 min and solvent quantity of 10 time

chlorogenic acid was linear in the range of 0.045-0.45 μg

regress equation was Y=2 329.028 9X+8.184 1（r=0.999 9）

the average recovery was 97.89% with RSD of 3.14%.Conclusion: The method of HPLC determination is simple

reliable and accurate and can be used for the quality control of Mori Cortex.

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