Pachymaran Up-regulates Immunoglobulin A Response and CD80,CD86 Expression in Intestines of Mice

WANG Qing1; HU Ming-hua2; DONG Yan1; PAN Hua-xin1; ZHOU Lian1; WANG Pei-xun1

doi:10.13422/j.cnki.syfjx.2011.13.056

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Pachymaran Up-regulates Immunoglobulin A Response and CD80,CD86 Expression in Intestines of Mice

更新时间：2024-09-23

- Pachymaran Up-regulates Immunoglobulin A Response and CD80,CD86 Expression in Intestines of Mice
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 13, Pages: 127-129(2011)
- 作者机构：
  
  1. 广州中医药大学免疫研究室
  2. 无限极(中国)有限公司研发中心
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2011.13.056
  CLC： R285.5
- Published：2011
- 稿件说明：
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[1]王青,胡明华,董燕,潘华新,周联,王培训.茯苓多糖对小鼠肠道分泌型免疫球蛋白A,CD80,CD86表达的影响[J].中国实验方剂学杂志,2011,17(13):127-129.

WANG Qing1, HU Ming-hua2, DONG Yan1, et al. Pachymaran Up-regulates Immunoglobulin A Response and CD80,CD86 Expression in Intestines of Mice[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(13): 127-129.
[1]王青,胡明华,董燕,潘华新,周联,王培训.茯苓多糖对小鼠肠道分泌型免疫球蛋白A,CD80,CD86表达的影响[J].中国实验方剂学杂志,2011,17(13):127-129. DOI： 10.13422/j.cnki.syfjx.2011.13.056.

WANG Qing1, HU Ming-hua2, DONG Yan1, et al. Pachymaran Up-regulates Immunoglobulin A Response and CD80,CD86 Expression in Intestines of Mice[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(13): 127-129. DOI： 10.13422/j.cnki.syfjx.2011.13.056.

摘要

目的:观察茯苓多糖对卵清白蛋白（OVA）诱导的小鼠肠道黏膜免疫应答的影响

探讨其对黏膜免疫的调节作用及其相关机制。方法:BALB/c小鼠随机分为2组:对照组和茯苓多糖组

对照组口服给予等体积生理盐水

茯苓多糖组每日按体重口服给药1次

剂量为200 mg·kg-1

2组小鼠给药1周后

1次性给予每只小鼠口服抗原卵清白蛋白（OVA）5 mg进行免疫

分别于免疫后1

3周

收集小鼠粪便样本

ELISA检测小鼠粪便OVA特异性分泌型免疫球蛋白A（sIgA）;2组小鼠给药1周后

分离小鼠派氏结

流式细胞术检测派氏结（Peyer’s patches）中B淋巴细胞共刺激分子CD80

CD86表达。结果:对照组小鼠粪便中OVA特异性sIgA在1

3周时分别为（19.39±2.89）

（17.60±5.65）

（14.48±4.55）μg·L-1

茯苓多糖组为（28.44±3.72）

（42.24±5.14）

（26.29±4.82）μg·L-1

与对照组比较有统计学意义（P<0.05）。对照组小鼠派氏结B细胞CD80

CD86表达率（%）分别为1.84±0.11和16.50±1.47;茯苓多糖组表达为2.76±0.29

22.02±1.89

高于对照组

有统计学意义（P<0.05）。结论:茯苓多糖对肠道sIgA分泌具有调节作用

这与其活化派氏结B淋巴细胞有关。

Abstract

Objective:To study the effect of pachymaran on the intestinal mucosal immune response in mice

and explore the molecular mechanism of the effect.Method: ELISA was used to detected the content of ovalbumin（OVA） specific sIgA in mice fecal

and the expression rate of CD80 and CD86 in Peyer’s patches were detected by flow cytometry

the change of them were also observed after administration of pachymaran.Result: Control mice showed only background levels of anti-OVA antibody.The levels of anti-OVA specific sIgA were increased by co-administration with pachymaran.And the expression rates of CD80 and CD86 in Peyer’s patches were increased significantly

compared with those of the control mice.Conclusion: The mucosal immunity against OVA was enhanced by co-administered with pachymaran

and the function of pachymaran was related to activation of B cell.

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