Regulation of Scorpio and Scolopendra on Intestinal Mucosal Immunity System in Rats with Collagen Induced Arthritis

LIU Duan-yong1; ZHAO Hai-mei1; CHENG Shao-min1; ZUO Zhi-qin1; LV Ai-ping3

doi:10.13422/j.cnki.syfjx.2011.13.058

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Regulation of Scorpio and Scolopendra on Intestinal Mucosal Immunity System in Rats with Collagen Induced Arthritis

更新时间：2024-09-23

- Regulation of Scorpio and Scolopendra on Intestinal Mucosal Immunity System in Rats with Collagen Induced Arthritis
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 13, Pages: 133-136(2011)
- 作者机构：
  
  1. 江西中医学院科技学院
  2. 江西中医学院中诊教研室
  3. 中国中医科学院中医临床基础研究所
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2011.13.058
  CLC： R285.5
- Published：2011
- 稿件说明：
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[1]刘端勇,赵海梅,程绍民,左志琴,吕爱平.全蝎、蜈蚣对胶原诱导型关节炎大鼠肠黏膜免疫状态的调节作用[J].中国实验方剂学杂志,2011,17(13):133-136.

LIU Duan-yong1, ZHAO Hai-mei1, CHENG Shao-min1, et al. Regulation of Scorpio and Scolopendra on Intestinal Mucosal Immunity System in Rats with Collagen Induced Arthritis[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(13): 133-136.
[1]刘端勇,赵海梅,程绍民,左志琴,吕爱平.全蝎、蜈蚣对胶原诱导型关节炎大鼠肠黏膜免疫状态的调节作用[J].中国实验方剂学杂志,2011,17(13):133-136. DOI： 10.13422/j.cnki.syfjx.2011.13.058.

LIU Duan-yong1, ZHAO Hai-mei1, CHENG Shao-min1, et al. Regulation of Scorpio and Scolopendra on Intestinal Mucosal Immunity System in Rats with Collagen Induced Arthritis[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(13): 133-136. DOI： 10.13422/j.cnki.syfjx.2011.13.058.

摘要

目的:观察全蝎、蜈蚣对胶原蛋白诱导型关节炎（collagen induced arthritis

CIA）大鼠肠黏膜免疫状态的调节作用。方法:Wistar大白鼠60只

随机分成6组

即正常组

模型组

全蝎蜈蚣高

中

低剂量组

Ⅱ型胶原蛋白（type Ⅱ collagen

CII）组。采用Ⅱ型胶原蛋白诱导法复制大鼠关节炎模型

并ig给予全蝎蜈蚣混悬液（0.4

0.2

0.1 g.kg-1）进行干预

CII组予CII蛋白冻干粉60μg.kg-1 ig进行对照

共计40 d。采用流式细胞术分别检测大鼠肠道派伊尔（peyer’s patches

PP）结淋巴细胞亚群水平

采用酶联免疫吸附试验法检测小肠组织匀浆转化生长因子β1（transforming growth factor-β1

TGF-β1）、分泌型免疫球蛋白（secreted immunoglobulin

sIgA）的表达水平。结果:与模型组比较

各治疗组均可明显下调CD4+

CD8+ T细胞水平

升高CD4/CD8水平

并具有显著性意义（P<0.05或P<0.01）。同时

除中剂量组外

其他各治疗组小肠组织匀浆sIgA水平均明显上升（P<0.01）

至于TGF-β1

则仅中剂量组和CII组小肠组织匀浆中TGF-β表达明显下降（P<0.05或P<0.01）。结论:全蝎、蜈蚣调节胶原免疫性关节炎黏膜免疫的作用可能通过调节小肠黏膜局部PP结T淋巴细胞亚群平衡

降低肠黏膜TGF-β1和升高sIgA表达水平来实现的。

Abstract

Objective:To observe regulation of scorpio and scolopendra on intestinal mucosal immunity system in rats with collagen induced arthritis（CIA）.Method: Sixty rats were randomly divided into 6 groups:normal control group

model control group

low-dose Scorpio and Scolopendra group

middle-dose Scorpio and Scolopendra group

high-dose Scorpio and Scolopendra group

and typeⅡcollagen group.Rheumatoid arthritis was induced by Collagen.Level of lymphocyte subsets from peyer’s patches was measured by flow cytometry

and expression of TGF-β1

sIgA in intestine was detected by enzyme linked immunosorbent assay（ELISA）.Result: Cellular level of CD4+

CD8+ was obviously descending in all treated groups when compared with the model group（P<0.05 or P<0.01）.Except for middle-dose Scorpio and Scolopendra group

while expression of sIgA in enteric homogenate from other treated groups was notably heightened（P<0.01）.On the contrary

TGF-β1 was remarkably lower in middle-dose Scorpio and Scolopendra group and type Ⅱcollagen group（P<0.05 or P<0.01）.Conclusion: Regulation of Scorpio and Scolopendra on intestinal mucosal immunity system in rats with collagen induced arthritis was potentially implemented by regulating balance of T-lymphocyte subsets

down-regulating expression of TGF-β1 and increasing sIgA expression in intestine.

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Related Author

LIU Duan-yong

ZHAO Hai-mei

CHENG Shao-min

ZUO Zhi-qin

LV Ai-ping

HUANG Xiao-ying

YANG Haoruo

KOU Qiuai

Related Institution

Xiyuan Hospital， China Academy of Chinese Medical Sciences

Tianjin University of Traditional Chinese Medicine

China Resources Sanjiu Medical & Pharmaceutical Co. Ltd.

National Resource Center for Chinese Materia Medica，China Academy of Chinese Medical Sciences

Guangxi University of Chinese Medicine

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