Effect of Genistein on Activity of Na+-K+-ATPase and Ca2+-Mg2+-ATPase in Rats with Myocardial Hypertrophy

HU Zhi-ping; HUANG Zhi-hua; WU Liang-liang; ZHENG Feng-xia; LI Liang-dong; ZENG Jing

doi:10.13422/j.cnki.syfjx.2011.13.065

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Effect of Genistein on Activity of Na⁺-K⁺-ATPase and Ca²⁺-Mg²⁺-ATPase in Rats with Myocardial Hypertrophy

更新时间：2024-09-23

- Effect of Genistein on Activity of Na⁺-K⁺-ATPase and Ca²⁺-Mg²⁺-ATPase in Rats with Myocardial Hypertrophy
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 13, Pages: 164-167(2011)
- 作者机构：
  
  赣南医学院
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2011.13.065
  CLC： R285.5
- Published：2011
- 稿件说明：
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[1]胡志苹,黄志华,吴亮亮,郑凤霞,李良东,曾靖.染料木素抗大鼠心肌肥厚作用及其与ATPase活性的关系[J].中国实验方剂学杂志,2011,17(13):164-167.

HU Zhi-ping, HUANG Zhi-hua, WU Liang-liang, et al. Effect of Genistein on Activity of Na⁺-K⁺-ATPase and Ca²⁺-Mg²⁺-ATPase in Rats with Myocardial Hypertrophy[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(13): 164-167.
[1]胡志苹,黄志华,吴亮亮,郑凤霞,李良东,曾靖.染料木素抗大鼠心肌肥厚作用及其与ATPase活性的关系[J].中国实验方剂学杂志,2011,17(13):164-167. DOI： 10.13422/j.cnki.syfjx.2011.13.065.

HU Zhi-ping, HUANG Zhi-hua, WU Liang-liang, et al. Effect of Genistein on Activity of Na⁺-K⁺-ATPase and Ca²⁺-Mg²⁺-ATPase in Rats with Myocardial Hypertrophy[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(13): 164-167. DOI： 10.13422/j.cnki.syfjx.2011.13.065.

摘要

目的:观察染料木素（Gen）对异丙肾上腺素（iso）所致大鼠心肌肥厚的保护作用及其对心肌组织Na+-K+-ATPase

Ca2+-Mg2+-ATPase活性及羟脯氨酸含量的影响。方法:0.2 g·L-1的iso 5 mL·kg-1·d-1

背部皮下注射

连续10 d

建立大鼠心肌肥厚模型。造模第2天开始给予不同浓度的染料木素、二甲基亚砜或NS

连续14 d

末次给药后禁食12 h

称体重

麻醉

取心脏

称全心及左心室质量

计算全心质量指数及左心室质量指数

测定心肌组织Na+-K+-ATPase

Ca2+-Mg2+-ATPase活性及羟脯氨酸（Hyp）含量。结果:模型组全心质量指数及左心室质量指数分别为（3.30±0.24）

（2.55±0.14）mg·kg-1

与正常对照组相比显著升高（P<0.001）;心肌组织Na+-K+-ATPase和Ca2+-Mg2+-ATPase活力为（1.81±0.25）

（1.00±0.22）U·mg-1

与正常对照组相比活性显著降低（P<0.01）;Hyp含量为（0.80±0.05）mg·g-1

与正常对照组相比含量显著升高（P<0.001）。与模型组相比

染料木素高、低剂量组（0.3

0.1μmol.kg-1）全心质量指数分别为（2.83±0.22）

（2.97±0.19）mg·kg-1;左心室质量指数分别为（2.32±0.16）

（2.34±0.13）mg·kg-1均显著降低（P<0.01或P<0.05）;高剂量组心肌组织Na+-K+-ATPase（2.58±0.40）U·mg-1及Ca2+-Mg2+-ATPase（1.35±0.22）U·mg-1活性升高（P<0.01或P<0.05）

染料木素高、低剂量组心肌组织Hyp含量分别为（0.48±0.11）

（0.57±0.14）mg·g-1显著降低（P<0.01或P<0.05）。结论:染料木素可通过提高Na+-K+-ATPase及Ca2+-Mg2+-ATPase的活性

降低Hyp含量

从而抑制心脏纤维化

起抗心肌肥厚作用。

Abstract

Objective:To observe the protection of genistein（Gen）on rat’s myocardial hypertrophy caused by isopropylarterenol（iso）

the changes in Na+-K+-ATPase and Ca2+-Mg2+-ATPase activities

and the content of hydroxyproline（Hyp）.Method: The rat’s myocardial hypertrophy model was formed through hypodermic injection which extended 10 days at back skin with iso

1 mg·kg-1·d-1.Once the model was formed

various doses of Gen

DMSO or NS were administrated through hypodermic injection from the second day

and the process will extend 14 days.After the last administration

the rats were fasted for 12 hours

and then hearts were taken under anesthesia.The indexes of heart and left ventricle were calculated

the concentration of Hyp as well as the activities of Na+-K+-ATPase and Ca2+-Mg2+-ATPase in myocardial tissue were investigated.Result: There was obvious difference between iso group and NS control group.Na+-K+-ATPase and Ca2+-Mg2+-ATPase activities in myocardial tissue of iso group were much weaker than those in the controls

while Hyp content

indexes of heart or left ventricle were increased.Administrated with Gen

those changes were reversed.Conclusion: Gen has a protection on rat’s myocardial hypertrophy caused by iso

and its mechanism probably comes from promoting Na+-K+-ATPase and Ca2+-Mg2+-ATPase activities and decreasing Hyp content.

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Related Author

HU Zhi-ping

HUANG Zhi-hua

WU Liang-liang

ZHENG Feng-xia

LI Liang-dong

ZENG Jing

ZHONG Sen-jie

XIONG Xia-jun

Related Institution

Institute of Traditional Chinese Medicine Diagnosis， Hunan University of Chinese Medicine

Research Institute of Traditional Chinese Medicine(TCM), Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of TCM

Guiyang University of Traditional Chinese Medicine

Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences

First Affiliated Hospital,Gannan Medical University

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