Influence of Jielv Anshen Tang on a Mouse Model of Social Isolation-induced Stress:Possible Insights into a Allopregnanolone Mechanism of Action in Anxiety Disorders

ZHOU Ning-na; HE Xiao-shan; CAO Dong; DAI Rong; TANG Dang-zhu

doi:10.13422/j.cnki.syfjx.2011.13.071

您当前的位置：

首页 >

文章列表页 >

Influence of Jielv Anshen Tang on a Mouse Model of Social Isolation-induced Stress:Possible Insights into a Allopregnanolone Mechanism of Action in Anxiety Disorders

更新时间：2024-09-23

- Influence of Jielv Anshen Tang on a Mouse Model of Social Isolation-induced Stress:Possible Insights into a Allopregnanolone Mechanism of Action in Anxiety Disorders
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 13, Pages: 185-188(2011)
- 作者机构：
  
  云南中医学院
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2011.13.071
  CLC： R285.5
- Published：2011
- 稿件说明：
移动端阅览
[1]周宁娜,何晓山,曹东,代蓉,唐当柱.解虑安神汤对社会隔离焦虑小鼠行为学及脑内ALLO水平的影响[J].中国实验方剂学杂志,2011,17(13):185-188.

ZHOU Ning-na, HE Xiao-shan, CAO Dong, et al. Influence of Jielv Anshen Tang on a Mouse Model of Social Isolation-induced Stress:Possible Insights into a Allopregnanolone Mechanism of Action in Anxiety Disorders[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(13): 185-188.
[1]周宁娜,何晓山,曹东,代蓉,唐当柱.解虑安神汤对社会隔离焦虑小鼠行为学及脑内ALLO水平的影响[J].中国实验方剂学杂志,2011,17(13):185-188. DOI： 10.13422/j.cnki.syfjx.2011.13.071.

ZHOU Ning-na, HE Xiao-shan, CAO Dong, et al. Influence of Jielv Anshen Tang on a Mouse Model of Social Isolation-induced Stress:Possible Insights into a Allopregnanolone Mechanism of Action in Anxiety Disorders[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(13): 185-188. DOI： 10.13422/j.cnki.syfjx.2011.13.071.

摘要

目的:通过观察解虑安神汤乙酸乙酯提取部位（J-A）对焦虑症动物模型行为学及脑内γ-氨基丁酸受体A亚型（GABAA-R）正性变构剂3α

5α-四氢孕酮（allopregnanolone

ALLO）的影响

探讨其抗焦虑的作用机制。方法:雄性小鼠随机分成隔离组和群养组。群养组每笼10只群养

作为正常对照组;隔离组小鼠单笼单只隔离饲养

复制社会隔离焦虑模型

随机分为正常对照组[0.5%聚氧乙烯山梨糖醇酐单油酸酯（吐温-80）]、模型组（0.5%吐温-80）、地西泮组（0.002 g·kg-1）、解虑安神汤乙酸乙酯提取部位组（J-A

0.21 g·kg-1）。从造模第3周开始ig给药

造模4周

于末次给药后1 h用高架十字迷宫（EPM）进行行为学测定

记录小鼠在EPM开臂、闭臂停留时间

进入开臂、闭臂次数

计算开放臂进入次数比例（OE%）及开放臂停留时间比例（OT%）;行为学实验结束后

测定脑内ALLO的含量。结果:与模型组相比

J-A组可缩短小鼠在闭臂停留时间（186.14±50.86）s

（245.47±35.00）s、增加进入开臂次数的百分比（19.38±15.57）%

（7.34±9.68）%

差异有显著性意义（P<0.05）;并具有延长小鼠在开臂停留时间

增加小鼠进入开臂次数、减少进入闭臂次数的趋势。脑内ALLO水平测定表明

与模型组相比

J-A组可增加小鼠脑内ALLO的水平

A依次为（0.90±0.13）

（0.77±0.11）

差异有显著性意义（P<0.05）。结论:解虑安神汤乙酸乙酯提取部位可能通过增加脑内ALLO的水平从而增强GABAA-R的功能发挥抗焦虑作用。

Abstract

Objective:By determining effects of the active compounds of acetoacetate extraction of Jielv Anshentang（JLAST）

on the Allopregnanolone（ALLO）

a positive allosteric modulator of the action of GABA at GABAA receptors in anxious animal model to elucidate its possible mechanism of antianxiety.Method: Socially isolated male mice were administrated with JLAST（0.21 g·kg-1）for two weeks

the elevated plus maze test（EPM）was performed

and then the ALLO in brain was detected.Result: JLAST could increase the downregulated ALLO in brain of the socially isolated male mice and attenuate anxiety-like behavior in EPM by increasing the percentage of open arm entries（OE%）

decreasing the close arm time;and showed a tendency to increase open arm time and entry.Conclusion: These data suggest that to normalize the downregulated ALLO and then enhence the function of GABAA-R might be the mechanism of action for the traditional Chinese medicine for treatment of anxiety disorders.

关键词

Keywords

references

Views

270

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Anxiolytic-like Effect of Active Phenolic Components from Moutan Cortex in Kunming Mice Animal Models

Related Author

ZHOU Ning-na

HE Xiao-shan

CAO Dong

DAI Rong

TANG Dang-zhu

SUN Shi-guang

Liu Jian

HAN Ying-hua

Related Institution

No data

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰