Effect of Quercetin on Growth and Apoptosis of Human Nasopharyngeal Carcinoma CNE2 Cells

GU Sheng-jiu; YANG Na; ZHU Kai-mei

doi:10.13422/j.cnki.syfjx.2011.13.073

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Effect of Quercetin on Growth and Apoptosis of Human Nasopharyngeal Carcinoma CNE2 Cells

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- Effect of Quercetin on Growth and Apoptosis of Human Nasopharyngeal Carcinoma CNE2 Cells
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 13, Pages: 192-194(2011)
- 作者机构：
  
  桂林医学院药学院
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2011.13.073
  CLC： R285.5
- Published：2011
- 稿件说明：
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[1]顾生玖,杨娜,朱开梅.槲皮素抑制人鼻咽癌CNE2细胞生长并诱导其凋亡的研究[J].中国实验方剂学杂志,2011,17(13):192-194.

GU Sheng-jiu, YANG Na, ZHU Kai-mei. Effect of Quercetin on Growth and Apoptosis of Human Nasopharyngeal Carcinoma CNE2 Cells[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(13): 192-194.
[1]顾生玖,杨娜,朱开梅.槲皮素抑制人鼻咽癌CNE2细胞生长并诱导其凋亡的研究[J].中国实验方剂学杂志,2011,17(13):192-194. DOI： 10.13422/j.cnki.syfjx.2011.13.073.

GU Sheng-jiu, YANG Na, ZHU Kai-mei. Effect of Quercetin on Growth and Apoptosis of Human Nasopharyngeal Carcinoma CNE2 Cells[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(13): 192-194. DOI： 10.13422/j.cnki.syfjx.2011.13.073.

摘要

目的:探讨槲皮素对人鼻咽癌CNE2细胞生长与凋亡的作用。方法:应用台盼蓝拒染法检测20

80μmol·L-1浓度槲皮素作用细胞24

72 h后的生长状况

流式细胞仪检测在以上浓度下作用细胞48 h后的细胞凋亡率

Western blot法检测以上浓度槲皮素作用细胞48 h后Bcl-2蛋白的表达情况。结果:各浓度槲皮素对人鼻咽癌CNE2细胞的生长有显著的抑制作用

呈明显的时间和剂量依赖性;经不同浓度的槲皮素作用48 h后

细胞凋亡率显著增加

当浓度在40μmol·L-1以上时

与对照组相比

差异具有统计学意义（P<0.01）;Western blot法检测Bcl-2蛋白的表达随着槲皮素浓度的增加而降低

槲皮素浓度在40μmol·L-1以上时

与对照组相比

差异具有统计学意义（P<0.01）。结论:槲皮素可以有效抑制人鼻咽癌CNE2细胞生长

诱导细胞凋亡

其诱导该细胞凋亡的作用机制可能是通过下调Bcl-2蛋白的表达。

Abstract

Objective:To investigate the effects of quercetin on growth and apoptosis of nasopharyngeal carcinoma cell line CNE2.Method:Using trypan blue exclusive assay

cell growth which were affected by 20

80 μmol·L-1 concentrations of quercetin were evaluated after 24

72 h.Mean time

the percentage of apoptotic cell was detected by FCM after 48 h.The expression of protein Bcl-2 was analyzed by western blot.Result:Inhibition rate of the nasopharyngeal cancer cell was obviously enhanced along with increase of the quercetin concentration

the effect was also showed in time course.Flow cytometry study revealed

with the concentration increased especially the concentration over 40 μmol·L-1

the percentage of apoptotic cells raised after 48h compared with the control group.The expression of protein Bcl-2 was down-regulated along with increase in quercetin concentration.When the concentration over 40 μmol·L-1

the number was significantly different compared with that of the control group.Conclusion:Quercetin can effectively inhibit tumor cell growth and induce tumor cells into apoptosis.It may be associate with the low expression of protein Bcl-2 in nasopharyngeal carcinoma cell line CNE2.

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Related Institution

Hunan Provincial Key Laboratory for the Prevention and Treatment of Ophthalmology and Otolaryngology Diseases with Traditional Chinese Medicine （TCM），Hunan University of Chinese Medicine

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College of Integrated Chinese and Western Medicine， Hunan University of Chinese Medicine

College of Medicine， Hunan University of Chinese Medicine

Chinese Medicine Hospital of Hainan Province

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