New Extraction Technology of Polysaccharide from Sea Cucumber

ZHAN Xue-kong1; ZHOU Hai-mei1; MA Xiao-hua2; LIU Quan-sheng1

doi:10.13422/j.cnki.syfjx.2011.15.001

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New Extraction Technology of Polysaccharide from Sea Cucumber

更新时间：2024-09-23

- New Extraction Technology of Polysaccharide from Sea Cucumber
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 15, Pages: 40-42(2011)
- 作者机构：
  
  1. 1. 海南全星医药研究院
  2. 海南椰岛(集团)股份有限公司
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2011.15.001
  CLC： TQ461
- Published：2011
- 稿件说明：
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[1]展学孔,周海妹,马小花,刘全胜.海参多糖提取新工艺[J].中国实验方剂学杂志,2011,17(15):40-42.

ZHAN Xue-kong1, ZHOU Hai-mei1, MA Xiao-hua2, et al. New Extraction Technology of Polysaccharide from Sea Cucumber[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(15): 40-42.
[1]展学孔,周海妹,马小花,刘全胜.海参多糖提取新工艺[J].中国实验方剂学杂志,2011,17(15):40-42. DOI： 10.13422/j.cnki.syfjx.2011.15.001.

ZHAN Xue-kong1, ZHOU Hai-mei1, MA Xiao-hua2, et al. New Extraction Technology of Polysaccharide from Sea Cucumber[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(15): 40-42. DOI： 10.13422/j.cnki.syfjx.2011.15.001.

摘要

目的:优选海参多糖的最佳提取工艺。方法:采用正交试验法

对提取海参多糖的影响因素进行研究

并以其含量为评价指标。结果:海参多糖的最佳提取工艺为以2倍体积的3%NaOH溶液进行提取后

再以2%的胰蛋白酶（酶解条件:pH 8.0

温度40℃

2 h）及2%的胃蛋白酶（酶解条件:pH 4.0

温度40℃

2 h）进行双酶水解

测得海参多糖质量分数达2.51%。结论:该提取工艺方法简单易行

重复性好

具可行性

可用于海参多糖的提取。

Abstract

Objective:To optimize the best extraction of sea cucumber polysaccharide.Method:Orthogonal test was applied to study the sea cucumber polysaccharide extracted factors with its content as evaluation index.Result:The best extraction of sea cucumber polysaccharide was as follows:2 times the volume of 3% NaOH solution was extracted

and then 2% trypsin（hydrolysis conditions:pH 8.0

temperature 40 ℃

time 2 h） and 2% pepsin（hydrolysis conditions:pH 4.0

temperature 40 ℃

time 2 h） were used for dual enzymatic hydrolysis;the content of sea cucumber polysaccharide measured was 2.51%.Conclusion:The extraction method is simple

reproducible and feasible;it can be used for sea cucumber polysaccharide extraction.

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Related Author

ZHAN Xue-kong

ZHOU Hai-mei

MA Xiao-hua

LIU Quan-sheng

Ren-guang WANG

Ai-ling JIA

Zhi-dong QIU

Jing-yao YANG

Related Institution

College of Pharmacy, Chengdu University of TCM

Key Laboratory of Active Ingredients in Traditional Chinese Medicine(TCM) Co-constructed by Jilin Province and Ministry of Education, Changchun University of Chinese Medicine

Research and Development Centre of New Drugs,Guangxi University of Chinese Medicine

Guangxi Bonger Pharmaceuticals Co.Ltd

Dongguan Hospital of Traditional Chinese Medicine

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