Analgesic Effect and Extraction,Purification Procedure of Ligusticum chuanxiong from Dachuanxiong Fang

ZHENG Qin; WU Zhen-feng; QIU Ling-ling; WEI Shao-feng; YUE Peng-fei; HU Peng-yi; YANG Ming

doi:10.13422/j.cnki.syfjx.2011.15.015

您当前的位置：

首页 >

文章列表页 >

Analgesic Effect and Extraction,Purification Procedure of Ligusticum chuanxiong from Dachuanxiong Fang

更新时间：2024-09-23

- Analgesic Effect and Extraction,Purification Procedure of Ligusticum chuanxiong from Dachuanxiong Fang
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 15, Pages: 6-9(2011)
- 作者机构：
  
  江西中医学院现代中药制剂教育部重点实验室
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2011.15.015
  CLC： R285.5
- Published：2011
- 稿件说明：
移动端阅览
[1]郑琴,伍振峰,邱玲玲,魏韶锋,岳鹏飞,胡鹏翼,杨明.大川芎方中川芎提取纯化工艺及其镇静镇痛作用[J].中国实验方剂学杂志,2011,17(15):6-9.

ZHENG Qin, WU Zhen-feng, QIU Ling-ling, et al. Analgesic Effect and Extraction,Purification Procedure of Ligusticum chuanxiong from Dachuanxiong Fang[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(15): 6-9.
[1]郑琴,伍振峰,邱玲玲,魏韶锋,岳鹏飞,胡鹏翼,杨明.大川芎方中川芎提取纯化工艺及其镇静镇痛作用[J].中国实验方剂学杂志,2011,17(15):6-9. DOI： 10.13422/j.cnki.syfjx.2011.15.015.

ZHENG Qin, WU Zhen-feng, QIU Ling-ling, et al. Analgesic Effect and Extraction,Purification Procedure of Ligusticum chuanxiong from Dachuanxiong Fang[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(15): 6-9. DOI： 10.13422/j.cnki.syfjx.2011.15.015.

摘要

目的:探讨大川芎方中川芎的提取纯化工艺条件。方法:分别采用回流法提取、大孔树脂法纯化川芎提取物

用高效液相色谱法测定提取物中阿魏酸的含量

采用小鼠镇痛、镇静试验验证提取物的药效。结果:川芎的最佳提取工艺为乙醇体积分数为70%

溶剂用量为8倍量

提取次数为3次

时间为每次2 h;提取液采用HPD100型大孔树脂纯化;纯化条件为上样液浓度0.5 g.mL-1

洗脱剂为6倍柱体积50%乙醇。药效试验表明提取物具有较好的镇静与镇痛效果。结论:提取纯化方法简便、准确

可为大川芎方现代制剂的开发提供技术参考。

Abstract

Objective:To study the extraction and purification method for Ligusticum chuanxiong Hort.in Dachuanxiong Fang.Method:Refluxing extract and macroporous adsorption resin were used for extraction and purification.HPLC method was used for the determination of ferulic acid.Using pharmacodynamic test validated the effect of extract.Result:The optmial extraction conditions were as follows:alcohol concentration 70%

extraction time 2 hours

8 of solid-to-liquid ratio and extracted 3 times.HPD100 was selected as the adsorbed resin

the concentration of extract sample was 0.5 g·mL-1

6 BV 50% alcohol was chose as elution agent.The result of pharmacodynamic test indicated the extract had a good effect on sedative and analgesia.Conclusion:The method is simple and accurate

which could provide technology reference for the development of modern preparation of Dachuanxiong Fang.

关键词

Keywords

references

Views

620

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Combination of Components from Tripterygii Radix et Rhizoma-Chuanxiong Rhizoma Affects RA-FLSs by Regulating NF-κB， Nrf2/HO-1 Signaling Pathways and Bcl-2/Caspase-3 Expression

Cloning and Expression Patterns of Coumarate-3-hydroxylase Gene from Angelica sinensis and Its Correlation with Content of Ferulic Acid

Chronic Toxicity of Triptolide and Ferulic Acid Ethosomes Gel for Percutaneous Administration

Metabonomics Analysis on Different Varieties of Angelica sinensis Based on UPLC-Q-TOF-MS

Related Author

ZHENG Qin

WU Zhen-feng

QIU Ling-ling

WEI Shao-feng

YUE Peng-fei

HU Peng-yi

YANG Ming

GUAN Yongmei

Related Institution

Laboratory Animal Science and Technology Center，Jiangxi University of Chinese Medicine

National Key Laboratory of Modern Chinese Medicine Creation of Classic Prescriptions

Jiangxi University of Chinese Medicine，Key Laboratory of Modern Preparation of Traditional Chinese Medicine，Ministry of Education

School of Life Science And Technology， Wuhan Polytechnic University

Key Laboratory of Modern Preparation of Traditional Chinese Medicine Under Ministry of Education， Jiangxi University of Chinese Medicine

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰