Optimization of Extraction Technology of Polysaccharide from Dendrobium nobile and Effects on Pro-liferation of Mouse Splenocytes

CHEN Zhi-guo1; YE Song-han1; FAN Ying1; HOU Jun-ran1; BIAN Hua1; PENG Yong-hong2

doi:10.13422/j.cnki.syfjx.2011.15.021

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Optimization of Extraction Technology of Polysaccharide from Dendrobium nobile and Effects on Pro-liferation of Mouse Splenocytes

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- Optimization of Extraction Technology of Polysaccharide from Dendrobium nobile and Effects on Pro-liferation of Mouse Splenocytes
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 15, Pages: 27-32(2011)
- 作者机构：
  
  1. 南阳理工学院国医学院
  2. 华南师范大学生命科学学院广东省植物发育生物工程重点实验室
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2011.15.021
  CLC： R285.5
- Published：2011
- 稿件说明：
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[1]陈志国,叶松山,范迎,侯俊然,卞华,彭永宏.金钗石斛多糖提取工艺的优化及对小鼠脾细胞增殖的影响[J].中国实验方剂学杂志,2011,17(15):27-32.

CHEN Zhi-guo1, YE Song-han1, FAN Ying1, et al. Optimization of Extraction Technology of Polysaccharide from Dendrobium nobile and Effects on Pro-liferation of Mouse Splenocytes[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(15): 27-32.
[1]陈志国,叶松山,范迎,侯俊然,卞华,彭永宏.金钗石斛多糖提取工艺的优化及对小鼠脾细胞增殖的影响[J].中国实验方剂学杂志,2011,17(15):27-32. DOI： 10.13422/j.cnki.syfjx.2011.15.021.

CHEN Zhi-guo1, YE Song-han1, FAN Ying1, et al. Optimization of Extraction Technology of Polysaccharide from Dendrobium nobile and Effects on Pro-liferation of Mouse Splenocytes[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(15): 27-32. DOI： 10.13422/j.cnki.syfjx.2011.15.021.

摘要

目的:优化金钗多糖的提取工艺

并研究其对小鼠脾细胞增殖的影响。方法:采用单因素试验和L9（34）正交试验法考察了提取时间、提取温度和料液比对金钗石斛多糖得率的影响

并用MTS/PMS法测定其对小鼠脾细胞增殖的影响。结果:金钗石斛多糖的最佳提取工艺条件为液料比45∶1

提取温度90℃

提取时间2.5 h;金钗石斛精制多糖、金钗石斛粗制多糖能直接促进脾细胞的增殖

且两者能显著增强刀豆蛋白A（ConA）诱导的T淋巴细胞和脂多糖（LPS）诱导的B淋巴细胞的增殖

在此最佳工艺条件下金钗石斛粗多糖得率为2.80%。结论:金钗石斛多糖能有效的促进脾淋巴细胞的增殖

并与Con A

LPS对T

B淋巴细胞的增殖有协同刺激作用。

Abstract

Objective:To optimize extraction technology of polysaccharides from Dendrobium nobile and to measure its effect on the splenocyte proliferation.Method:Based on the single factors test

such as extraction time

extraction temperature and solvent to material ratio were optimized by orthogonal test L9（34）.MTS/PMS assay was used to measure the splenocyte proliferation.Result:The optimal technology conditions were as follows:extraction temperature was set at 90 ℃

and extraction time was 2.5 h with ratio of 45:1.D.nobile crude polysaccharide（DNCP） and D.nobile purificatory polysaccharide（DNPP） resulted in a significant increase of lymphocyte proliferation.DNCP and DNPP could also enhance the effect of Con A-induced T lymphocyte proliferation and the stimulation index of LPS-induced B lymphocytes.Conclusion:Under these conditions the polysaccharides yield of D.nobile was up to 2.80%.DNCP and DNPP can effectively increase lymphocyte proliferation and enhance the effect of Con A

LPS-induced T and B cell growth.

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