ZHANG Jun1, LIU Yu-zheng1, WANG Yue-sheng1, et al. Simultaneous Determination of 12 Active Components In Gegenqinlian Decoction by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(15): 58-62.
ZHANG Jun1, LIU Yu-zheng1, WANG Yue-sheng1, et al. Simultaneous Determination of 12 Active Components In Gegenqinlian Decoction by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(15): 58-62. DOI: 10.13422/j.cnki.syfjx.2011.15.029.
Simultaneous Determination of 12 Active Components In Gegenqinlian Decoction by HPLC
Objective:To establish a HPLC-DAD method for simultaneous determination of puerarin
daidzin
liquiritin
coptisine hydrochloride
jatrorrhizine hydrochloride
baicalin
berberine hydrochloride
palmatine hydrochloride
wogonoside
baicalein
wogonin and ammonium glycyrrhizinate in Gegenqinlian decoction.Method:A Merck Chromolith RP-18e(4.6 mm × 100 mm) was used;the mobile phase was acetonitrile-0.05% phosphoric acid(contain 0.01 mol.L-1 potassium dihydrogen phosphate) in gradient elution.The detection wavelength was set at 250
275
345 nm
the flow rate was 2.0 mL.min-1 and the column temperature was maintained at 35 ℃.Result:The accuracy
precision
sensitivity
specificity and linearity of this method met the requirements.The content of the twelve active components was determined simultaneously.Conclusion:The method is rapid
simple and accurate
it has a good specificity and it can be suitable for the determination of twelve active components in Gegenqinlian decoction simultaneously.
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Related Author
ZHANG Jun
LIU Yu-zheng
WANG Yue-sheng
LIU An
LI Hongrong
ZHANG Shixiong
LI Yawen
LU Xuan
Related Institution
Hebei Institute of Integrated Traditional Chinese and Western Medicine
National Key Laboratory for Innovation and Transformation of Luobing Theory
Hebei Yiling Hospital
Key Research Laboratory of National Administration of Traditional Chinese Medicine(Luobing of Cardio-cerebral Vessel), Hebei Yiling Medical Research Institute
Pharmaceutical College,Shandong University of Traditional Chinese Medicine