BI Wei1, XING Yan-yi2, LI Yan-yan2, et al. Study on Content Determination of Peptide in Trionycis Carapax by Biuret Reagent[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(15): 63-65.
BI Wei1, XING Yan-yi2, LI Yan-yan2, et al. Study on Content Determination of Peptide in Trionycis Carapax by Biuret Reagent[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(15): 63-65. DOI: 10.13422/j.cnki.syfjx.2011.15.030.
Study on Content Determination of Peptide in Trionycis Carapax by Biuret Reagent
摘要
目的:用双缩脲试剂显色
可见分光光度法测定醋鳖甲中的总肽含量。方法:显色剂为0.02 g.mL-1的酒石酸钠钾、5%的NaOH溶液0.3 mL、1%的CuSO4溶液1 mL
检测波长545 nm。结果:七肽在2.50~10.04 mg与吸收度呈良好的线性关系
精密度、重现性、稳定性均合乎要求
平均回收率为98.02%
RSD为1.65%(n=5)
符合要求。结论:实验首次使用该方法测定中药中的肽类成分
创新性强
简便可行并且精确度高。
Abstract
Objective:To determine the content of peptide using biuret reagent and VIS-spectrophotometric method.Method:The developer is 0.02 g·mL-1 sodium potassium tartrate solution(1 mL)
5%NaOH(0.3 mL)
1%CuSO4(1 mL).The wave length of detection is at 545 nm.Result:The calibration curves are linear at in the ranges of 2.5-10.04 mg for septa-peptide(r=0.999 7).The average recovery is 98.02%