QIN Jie-ping1, YAO Rong2, LI Yao-hua1. Study on HPLC-ELSD Fingerprint of Butanol Extracts of Fufang Fufangteng Preparation[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(15): 72-75.
QIN Jie-ping1, YAO Rong2, LI Yao-hua1. Study on HPLC-ELSD Fingerprint of Butanol Extracts of Fufang Fufangteng Preparation[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(15): 72-75. DOI: 10.13422/j.cnki.syfjx.2011.15.033.
Study on HPLC-ELSD Fingerprint of Butanol Extracts of Fufang Fufangteng Preparation
摘要
目的:建立复方扶芳藤制剂正丁醇部位HPLC-ELSD指纹图谱的检测方法
为该制剂的皂苷类成分指纹图谱质量控制提供新的方法。方法:采用Shim-Pack CLC-ODS色谱柱(6.0 mm×150 mm
Objective:To establish the mutual mode of HPLC-ELSD fingerprint of butanol extracts of Fufang Fufangteng preparation
by which a new approach to assessing the quality of this medicine is provided.Method:A Shim-Pack CLC-ODS column(6.0 mm×150 mm i.d.
5 μm) with a gradient elution of acetonitrile and water was used
the flow rate was set at 1 mL·min-1.Ten batches of certified products were selected to establish the mutual mode of HPLC fingerprint of Fufangteng preparation by matching chromatographic patterns and correcting retention times.Result:This mutual mode of HPLC fingerprint can distinguish the certified Fufangteng preparation products from others by contrasting with their HPLC fingerprints.Conclusion:This method can provide more information for the quality control of Fufang Fufangteng and be used for the quality control of the saponins in this preparation products.
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Related Author
QIN Jie-ping
YAO Rong
LI Yao-hua
CHEN Aijun
HUANG Siqi
LIU Xiaoxuan
TANG Yu
ZHANG Fanghua
Related Institution
Hunan Polytechnic of Environment and Biology
School of Pharmacy,Hunan Provincial Key Laboratory of Drugability and Preparation Modification of Traditional Chinese Medicine,Hunan University of Chinese Medicine
Affiliated Hospital of Hunan Academy of Chinese Medicine
Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing University of Chinese Medicine