NIU Xiao-jing, SHI Jun-han. Simultaneous Determination of Loganin and Puerain in Anke Capsule by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(15): 90-92.
NIU Xiao-jing, SHI Jun-han. Simultaneous Determination of Loganin and Puerain in Anke Capsule by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(15): 90-92. DOI: 10.13422/j.cnki.syfjx.2011.15.038.
Simultaneous Determination of Loganin and Puerain in Anke Capsule by HPLC
Objective:To establish the method for the assaying of loganin and puerain in Anke capsule with HPLC method.Method:The chromatographic column was Aglient Eclipse XDB-C18.The mobile phase was consisted of acetonitrile-water(12:88).The detection wavelength was 240 nm and 250 mm.The flow rate was 1.0mL.min-1.The injection volume was 10 μL.Result:Loganin was linear in the range of 0.31-2.175 μg(r =0.999 9).Puerarin was a linear in the range of 0.164-1.148 μg(r=0.999 9).The average recovery of loganin was 100.39% and RSD was 0.72%(n=6).The average recovery of puerain was 97.99% and RSD was 1.98%(n=6).Conclusion:This method was simple and specificity with good repeatability
it can be used for the quality control of Anke capsule.
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Related Author
NIU Xiao-jing
SHI Jun-han
GONG Wei-hong
ZHAO Xi-xian
shijunhan
SHI Jun-han
NIU Xiao-jing
ZHAO Xin-hong
Related Institution
First Affiliated Hospital of Henan University of Traditional Chinese Medicine
Beijing University of Chinese Medicine,School of Chinese Materia Medica
Chongwen District,Beijing Institute for Drug Control
College of Pharmacy, Shaanxi University of Chinese Medicine
National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicine, Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences