Effective Components of Zhuanggu Zhitong Prescription and its Influence on Hormones Related to Bone Metabolism in Female Rats

GAN Guo-xing1; MO Xin-min1; LI Jing-ping2; ZHANG Guo-min1; FAN Juan-juan1; LIU Shi-jie1

doi:10.13422/j.cnki.syfjx.2011.15.050

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Effective Components of Zhuanggu Zhitong Prescription and its Influence on Hormones Related to Bone Metabolism in Female Rats

更新时间：2024-09-23

- Effective Components of Zhuanggu Zhitong Prescription and its Influence on Hormones Related to Bone Metabolism in Female Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 15, Pages: 126-130(2011)
- 作者机构：
  
  1. 湖南中医药大学
  2. 中南大学药学院
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2011.15.050
  CLC： R285.5
- Published：2011
- 稿件说明：
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[1]甘国兴,莫新民,李劲平,张国民,范娟娟,刘仕杰.壮骨止痛方药效部位的筛选及其对骨质疏松雌鼠骨代谢相关激素的影响[J].中国实验方剂学杂志,2011,17(15):126-130.

GAN Guo-xing1, MO Xin-min1, LI Jing-ping2, et al. Effective Components of Zhuanggu Zhitong Prescription and its Influence on Hormones Related to Bone Metabolism in Female Rats[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(15): 126-130.
[1]甘国兴,莫新民,李劲平,张国民,范娟娟,刘仕杰.壮骨止痛方药效部位的筛选及其对骨质疏松雌鼠骨代谢相关激素的影响[J].中国实验方剂学杂志,2011,17(15):126-130. DOI： 10.13422/j.cnki.syfjx.2011.15.050.

GAN Guo-xing1, MO Xin-min1, LI Jing-ping2, et al. Effective Components of Zhuanggu Zhitong Prescription and its Influence on Hormones Related to Bone Metabolism in Female Rats[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(15): 126-130. DOI： 10.13422/j.cnki.syfjx.2011.15.050.

摘要

目的:筛选壮骨止痛方的药效部位

研究其对骨代谢相关激素的影响

探讨其可能的作用机制。方法:选取3月龄250 g左右SD雌性大鼠150只

按体重随机分为模型组、假手术组、正常组、壮骨止痛方无水乙醇提取A部位组、无水乙醇提取B部位组

石油醚提取A部位组

水提A部位组

水提B部位组

乙酸乙酯提取A部位组

壮骨止痛方组

共10组

每组15只。去卵巢术后5 d拆线后开始给药

连续13周。取血和骨标本从骨密度、骨生物力学、病理形态学等几个方面研究其药效

从血清雌激素（E2）、骨钙素（BGP）、甲状旁腺素（PTH）、降钙素（CT）、瘦素、胰岛素方面研究其作用机制。结果:与模型组相比

壮骨止痛方无水乙醇提取A部位、水提B部位、石油醚提A部位能明显增加去卵巢模型鼠股骨、腰椎骨的骨密度（P<0.05或P<0.01）;能显著提高股骨头平均骨小梁密度

差异非常明显（P<0.01）

能显著增强去势骨质疏松模型大鼠胫骨的最大应力和最大抗弯强度（P<0.05或P<0.01）;与壮骨止痛方组比较无明显差异。与去势模型组比较

壮骨止痛方能显著增加血清E2

预防雌激素不足导致的子宫萎缩（P<0.05）;显著降低去势模型鼠血清BGP

PTH

CT（P<0.05）。结论:壮骨止痛方无水乙醇提取A部位、水提B部位、石油醚提A部位是壮骨止痛方治疗骨质疏松症的药效部位;壮骨止痛方能够增加去卵巢骨质疏松模型大鼠血清E2含量

降低血清BGP

PTH

直接或间接调节了机体的骨代谢和细胞因子

改善骨基质的分子结构

加强骨生物力学性能

从而治疗骨质疏松症。

Abstract

Objective:To find the effective components of Zhuanggu Zhitong prescription

investigate its influence on hormones related to bone metabolism and to explore its possible mechanism.Method:One hundred and fifty SD female rats weighing around 250 g（three months old） were randomly divided into model group（ovariectomized

OVX）

sham operation group

control group

ethanol extraction group A

ethanol extraction group B

petroleum ether extraction A position group

water extraction site group A

water extraction site group B

Fraction extracted with ethyl acetate group A

Zhuanggu Zhitong prescription group

a total of 10 groups（n=15 each）.Five days after stitches of the operation of taking out ovaries

different treatments were carried out for 13 weeks.Blood and bone samples were collected for investigations on bone mineral density

bone biomechanics

pathology and other aspects to evaluate efficacy

and serum estradiol（E2）

bone gla protein（BGP）

parathyroid hormone（PTH）

calcitionin（CT）

to explore the effective mechanism.Result:Compared with model group

Zhuanggu Zhitong prescription group A part of ethanol extraction

water extraction B parts

petroleum ether A site could significantly increase the levels of OVX rats femur

lumbar spine bone mineral density（P<0.05

P<0.01）;could increase osteoporosis in ovariectomized rat tibia model the maximum stress and maximum bending strength（P<0.05

P<0.01）;could significantly increase the average trabecular bone density of the femoral head

the difference was significant（P<0.01）

with no significant difference to Zhuanggu Zhitong prescription group.Compared to OVX model group

Zhuanggu Zhitong prescription could only significantly increase in serum E2

prevent uterine atrophy caused by insufficient estrogen（P<0.05）;Zhuanggu Zhitong prescription could significantly decrease serum BGP

PTH

CT for the castrated rats（P<0.05）.Conclusion:The ethanol extract parts of group A

water extraction site group B

petroleum ether extract A position group site are the effective components of Zhuanggu Zhitong prescription for treating osteoporosis.Zhuanggu Zhitong prescription can increase the serum level of E2

and decrease the levels of BGP

PTH

directly or indirectly regulate the body’s bone metabolism and cytokines

to improve hormone metabolism

the molecular structure of bone matrix and to strengthen the mechanical properties of bone

therefore to treat osteoporosis.

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Related Author

GAN Guo-xing

MO Xin-min

LI Jing-ping

ZHANG Guo-min

FAN Juan-juan

LIU Shi-jie

MENG Shilong

ZHANG Xu

Related Institution

TCM Hospital Xianju of the Second Affiliated Hospital of Zhejiang Chinese Medical University

The Second Affiliated Hospital of Zhejiang Chinese Medical University

The Second School of Clinical Medicine，Zhejiang Chinese Medical University

Dongzhimen Hospital， Beijing University of Chinese Medicine

Beijing University of Chinese Medicine

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