Protective Effect of Chlorogenic Acid on Liver Fibrosis in Rats

QI Xiao-yuan1; SHI Xiu-ling2; GAO Yin-hui2; WANG Mei2; WANG Xu2; ZHOU Cheng-yan2

doi:10.13422/j.cnki.syfjx.2011.15.053

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Protective Effect of Chlorogenic Acid on Liver Fibrosis in Rats

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- Protective Effect of Chlorogenic Acid on Liver Fibrosis in Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 15, Pages: 139-143(2011)
- 作者机构：
  
  1. 河北联合大学附属医院检验科
  2. 河北联合大学药学院
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2011.15.053
  CLC： R285.5
- Published：2011
- 稿件说明：
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[1]戚晓渊,史秀灵,高银辉,王美,王旭,周程艳.绿原酸抗肝纤维化作用的研究[J].中国实验方剂学杂志,2011,17(15):139-143.

QI Xiao-yuan1, SHI Xiu-ling2, GAO Yin-hui2, et al. Protective Effect of Chlorogenic Acid on Liver Fibrosis in Rats[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(15): 139-143.
[1]戚晓渊,史秀灵,高银辉,王美,王旭,周程艳.绿原酸抗肝纤维化作用的研究[J].中国实验方剂学杂志,2011,17(15):139-143. DOI： 10.13422/j.cnki.syfjx.2011.15.053.

QI Xiao-yuan1, SHI Xiu-ling2, GAO Yin-hui2, et al. Protective Effect of Chlorogenic Acid on Liver Fibrosis in Rats[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(15): 139-143. DOI： 10.13422/j.cnki.syfjx.2011.15.053.

摘要

目的:研究绿原酸对四氯化碳（CCl4）导致的肝纤维化大鼠的保护作用

为其进一步开发和研究提供科学依据。方法:大鼠随机分为6组

即正常组

模型组

秋水仙碱（0.2 mg.kg-1）组

绿原酸高

中

低剂量（140

35 mg.kg-1）组。各组每天ig给药

给药容量为20 mL.kg-1.d-1

连续8周。正常组和模型组给予蒸馏水。给药和造模同时进行。采用CCl4导致大鼠肝纤维化模型

除正常组外

其余各组每只大鼠首次于背部sc纯CCl4 5 mL.kg-1

以后sc 40%CCl4花生油3 mL.kg-1

共8周。第8周经股动脉取血

计算大鼠的肝脾指数

检测大鼠血清中丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）的活性及其总蛋白（TP）、白蛋白（ALB）的含量

计算ALB与球蛋白（GLOB）的比值;检测血清中透明质酸（HA）、层黏连蛋白（LN）、Ⅲ型前胶原（PCⅢ）、Ⅳ型胶原（CⅣ）的含量;检测肝脏组织中超氧化物歧化酶（SOD）的活性、羟脯氨酸（Hyp）的含量、丙二醛（MDA）和谷胱甘肽过氧化物酶（GSH-Px）的水平。取肝组织

做病理切片

HE和Masson染色。结果:模型组大鼠血清中的ALT

AST

ALB

A/G

肝纤维化4项指标（即PCⅢ

CⅣ

LN）均具有显著的肝纤维化的特征（P<0.01）。绿原酸能明显对抗CCl4所致的肝纤维化大鼠肝脾系数的升高（P<0.01）;显著对抗血清中ALT

AST活性的升高（P<0.01）

降低血清中HA

PCⅢ

CⅣ

GLOB的含量（P<0.01）

升高血清中的TP

ALB含量和A/G（P<0.01）;降低肝组织中MDA和Hyp的含量（P<0.01）

升高肝组织中SOD和GSH-Px的活性（P<0.01）。绿原酸各剂量组中绿原酸高剂量组抗肝纤维化的效果最好

并呈现明显的剂量依赖性。病理结果亦显示绿原酸具有良好的抗肝纤维化的作用。结论:绿原酸具有显著的抗肝纤维化的作用。

Abstract

Objective:To study the protective effect of chlorogenic acid on liver fibrosis in rats.Method:The rats were randomly divided into 6 groups:the normal control

the model group

the colchicina group（0.2 mg·kg-1）

and the chlorogenic acid groups（140

35 mg·kg-1）.Rats in the groups were treated daily for 8 weeks.Model of liver fibrosis induced by carbon tetrachlotide（CCl4） in rats was used.To establish the model

except the rats in the normal control group

the other mice were sc injected with CCl4（5 mL·kg-1） as the first attack and then sc injection of peanut oil of 40% CCl4（3 mL·kg-1） twice a week in the following 8 weeks.The indexes of live and spleen in rats were observed.After blood sample collection

alanine aminotransferase（ALT） and aspartate aminotransferase（AST） activities and content of total protein（TP）

albumin（ALB） in serum were examined

meanwhile the ratio of the ALB and globulin（GLOB） was calculated.The four indicators of liver fibrosis hyaluronic acid（HA）

laminin（LN）

procoliagen type Ⅲ（PCⅢ）

collagen type Ⅳ（CⅣ） were determined in liver tissue.Superoxide dismalase（SOD） activity and malondial-dehyde（MDA）

glutalhioneperoxidase（GSH-Px） levels in liver tissue were determined.Result:Chlorogenic acid could obviously decrease the index increase of the live and spleen（P<0.01） in liver fibrosis of rat

remarkably resist the increase of ALT and AST in serum（P<0.01）

increase the content of TP

ALB

A/G in serum（P<0.01）

suppress the content of HA

PCⅢ

CⅣ

GLOB in liver tissue（P<0.01）

decrease the MDA levels in liver tissue（P<0.01）

and improve SOD activity and GSH-Px level in liver tissue（P<0.01）.Conclusion:Chlorogenic acid has significant protective effect on liver fibrosis in rats.

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Related Author

QI Xiao-yuan

SHI Xiu-ling

GAO Yin-hui

WANG Mei

WANG Xu

ZHOU Cheng-yan

WANG Xiaojie

ZHANG Heng

Related Institution

The First Affiliated Hospital of Henan University of Chinese Medicine

The First Clinical Medical College of Henan University of Chinese Medicine

Henan General Hospital

Graduate School of Anhui University of Chinese Medicine

The First Affiliated Hospital of Anhui University of Chinese Medicine

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