Objective:To observe the safety on oil concoction of Sanhuangpian.Method:Acute toxicity test was made in 40 mice skin by smearing individually oil（0.2 mL per mice）on the bare skin of mice back for control group and the oil concoction of Sanhuangpian（0.4 g per mice）for treatment group.At same time we have made the skin irritation test through in 20 guinea pigs skin by smearing the oil concoction of Sanhuangpian（1.0 g per guinea pig）on the left bare skin of guinea pig back and oil（1.0 mL per guinea pig）on that of the right.Then the skin allergic test was made on 30 skin of guinea pig by smearing oil concoction of Sanhuangpian（0.5 g per guinea pig）on the bare skin of guinea pig back for treatment group

with oil（0.5 mL per guinea pig）and 2

4-dinitrochlorobenzene（0.5 mL guinea pig） respectively for control group.Result:The external application of Sanhuangpian oil concoction has no significant toxicity on skin

there was no animal death in the observing continuously of 7 days after using of Sanhuangpian oil concoction

theiy activities were not abnormal.There is no significant irritation on skin

no erythema of guinea pig skin

edema

allergic reactions

sensitization rates were 10%

0

there is no skin irritation obvious allergic reactions.Conclusion:The oil concoction of Sanhuangpian has definite safety on clinical application

a more widespread use should been made to people.